Adult koi carp (Cyprinus carpio L.) were fed with feed supplemented with vitamin C at 200 mg/kg feed (T1), 400 mg/kg feed (T2) and 600 mg/kg feed (T3), and vitamin E at 200 mg/kg feed (T4), 400 mg/kg feed (T5) and 600 mg/kg feed (T6) at ad libitum for 60 days. Control (without vitamin inclusion) was also maintained. Observations were made on the growth performance, GSI and biochemical composition of gonads such as crude protein, crude fat, cholesterol and carotenoid once in 15 days. At the end of the experiment, fertilization was done to record the fertilization and hatching rate. The difference in the values was noticed among the male and female koi carp. Fish supplemented with vitamin E at 200 mg/kg feeexhibited higher growth in both male and female fish, and the fertilization rate and hatching rate were also higher in that group, and the values were significantly different (p < 0.05) and showed linear and quadratic trends. Inclusion of vitamin C at 600 mg/kg feed had shown higher lipid and carotenoid contents of female koi carp (p<0.05). Hence, the results revealed that supplementing vitamin C and E in the diet is necessary for the successful reproduction of koi carp.

中文翻译：

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抗氧化剂对锦鲤（Cyprinus carpio L.）生长性能、性腺指数和性腺生化特性及受精成功率的影响

成年锦鲤 ( Cyprinus carpio L.) 的饲料中添加了 200 毫克/千克饲料 (T1)、400 毫克/千克饲料 (T2) 和 600 毫克/千克饲料 (T3) 的维生素 C，以及 200 毫克/千克饲料 (T3) 和维生素 E mg/kg 饲料 (T4)、400 mg/kg 饲料 (T5) 和 600 mg/kg 饲料 (T6)随意60 天。还保持对照（不含维生素）。每15天观察一次性腺的生长性能、GSI和生化成分，如粗蛋白、粗脂肪、胆固醇和类胡萝卜素。实验结束时进行施肥，记录受精和孵化率。在雄性和雌性锦鲤之间注意到了数值的差异。补充维生素E 200 mg/kg的鱼，雄鱼和雌鱼均表现出较高的生长速度，该组的受精率和孵化率也较高，且数值有显着差异（p  < 0.05），呈线性和二次曲线趋势。在饲料中添加 600 mg/kg 的维生素 C 显示雌性锦鲤的脂质和类胡萝卜素含量更高（p<0.05）。因此，结果表明，在饲料中补充维生素 C 和 E 是锦鲤成功繁殖的必要条件。