Journal of Applied Genetics ( IF 2.4 ) Pub Date : 2021-07-09 , DOI: 10.1007/s13353-021-00650-3 Jie Zhang 1 , Li-Mei Lin 1 , Wen-Wen Cheng 1 , Xin Song 1 , Yue-Hong Long 1 , Zhao-Bin Xing 1
Glycosyltransferase gene family 1, also known as uridine diphosphate glycosyltransferase (UGT), is the largest glycosyltransferase family in plants, playing a vital role in their growth and development. In this study, 244 UGT genes with conserved PSPG motifs were identified in the genome of Quercus robur L. The collinearity analysis results showed that tandem repeat was the main way of UGT genes expansion in Q. robur, with 21 groups of 55 tandem repeat genes. UGT genes were divided into 15 subgroups A–P; group K was lost, and the gene structure and conserved domain of the same subgroup were basically the same. Cis-element analysis showed that upstream 2,000 bp promoter sequence of UGT genes contained light response elements, plant hormone response elements, and stress-related cis-elements, which indicated that UGT genes of Q. robur might be regulated by various metabolic pathways. In particular, some UGTs in group L of Q. robur contained a conserved promoter structure. The expression pattern analysis results demonstrated that UGT genes of groups B, D, E, and I were differentially expressed under Tortrix viridana L. stress. The expression of UGTs in group E decreased under stress, the expression of group L increased, and that of genes in groups D and B were different. The functions of UGT genes in E and L groups are relatively conservative, and their functions may also conserve among species. The study results have a particular reference value for further research on the function of Q. robur UGT genes.
中文翻译:
栎属植物糖基转移酶基因家族1的全基因组鉴定及表达分析。
糖基转移酶基因家族1,也称为尿苷二磷酸糖基转移酶(UGT),是植物中最大的糖基转移酶家族,在植物的生长发育中起着至关重要的作用。在这项研究中,244个UGT基因与保守PSPG图案中的基因组进行鉴定夏栎属共线性分析结果表明,串联重复是的主要途径UGT基因中扩张Q.栎,拥有21组55串联重复基因. UGT基因分为15个亚组A-P;K组丢失,同一亚组的基因结构和保守结构域基本相同。顺式元件分析表明,UGT上游 2,000 bp 启动子序列基因中含有光反应元件、植物激素反应元件和逆境相关顺式元件,表明Q. robur的UGT基因可能受多种代谢途径的调控。特别是,Q. robur L 组中的一些UGT含有保守的启动子结构。表达模式分析结果表明,B、D、E和I组的UGT基因在Tortrix viridana L.胁迫下差异表达。的表达UGT下应力S E组下降,基团L的表达增加,并且在基团d和B的基因是不同的。UGT的功能E组和L组的基因相对保守,其功能在物种间也可能保守。该研究结果对进一步研究罗伯菌UGT基因的功能具有一定的参考价值。