Bovine parvovirus 1 (BPV1) is a causative agent of respiratory, gastrointestinal, and reproductive cattle diseases. We collected 149 yak diarrhea fecal samples from 9 farms in the Qinghai-Tibet Plateau. The samples were screened for BPV1 by PCR, and 2 samples were positive for BPV1. The complete genomes of these BPV1 isolates were sequenced successfully. The sequences of these 2 variants were both 5,515 bp in length and shared 96.5–96.8% identity with 2 previously reported BPV1 genomes (GenBank DQ335247, NC_001540). Twenty-six identical amino acid mutations were found in the 2 yak variants, including 7 amino acid substitutions in receptor-binding regions of the VP2 protein, and 5 amino acid substitutions in the NS1 protein C-terminal region that functions to activate transcription. The new genome sequences contribute to better understanding of the evolution and molecular characteristics of BPV1.

牛细小病毒 1 (BPV1) 是呼吸道、胃肠道和生殖牛疾病的病原体。我们收集了青藏高原 9 个农场的 149 份牦牛腹泻粪便样本。通过PCR对样本进行BPV1筛选，2个样本为BPV1阳性。这些 BPV1 分离株的完整基因组已成功测序。这 2 个变体的序列长度均为 5,515 bp，与之前报道的 2 个 BPV1 基因组（GenBank DQ335247，NC_001540）具有 96.5-96.8% 的同一性。在 2 个 yak 变体中发现了 26 个相同的氨基酸突变，包括 VP2 蛋白受体结合区的 7 个氨基酸替换，以及用于激活转录的 NS1 蛋白 C 端区域的 5 个氨基酸替换。