The aim of the study was to reveal the effect of neurostimulation with the TKPRPGP neuropeptide on the expression intensity of Doublecortin and Nestin in the olfactory bulb of white Wistar rats using immunohistochemical and computer analysis methods. An isolated assessment of early progenitor differentiation by the density of nestin-positive structures showed that stimulation from birth to 14 days preserves the level of nestin expression, preventing its decrease. When the administration of the neuropeptide is stopped, the expression of nestin decreases sharply, starting from the central zones of the bulb, and after three weeks it is no longer present. The dynamics of doublecortin positive structure density reflects an increase upon neuropeptide administration. Each course of neuropeptide administration caused an increase in the density of the marker, but the degree of effectiveness decreased with age, and the duration of the effect decreased. In conclusion, administration of the neuropeptide TKPRPGP to rats at an early age prolongs the expression of nestin and doublecortin in the olfactory bulbs of rats up to 35 days and up to 74 days of observation, respectively. The administration of the neuropeptide in adulthood does not lead to re-expression of these markers.

该研究的目的是使用免疫组织化学和计算机分析方法揭示 TKPRPGP 神经肽的神经刺激对白色 Wistar 大鼠嗅球中 Doublecortin 和 Nestin 表达强度的影响。通过巢蛋白阳性结构的密度对早期祖细胞分化的单独评估表明，从出生到 14 天的刺激保留了巢蛋白表达水平，防止其下降。当停止给予神经肽时，巢蛋白的表达急剧下降，从球茎的中心区域开始，三周后不再存在。双皮质素阳性结构密度的动态反映了神经肽给药后的增加。神经肽给药的每个疗程都会导致标记物的密度增加，但有效性程度会随着年龄的增长而降低，并且作用的持续时间也会缩短。总之，在大鼠早期给予神经肽 TKPRPGP 可将大鼠嗅球中巢蛋白和双皮质素的表达分别延长长达 35 天和长达 74 天的观察时间。在成年期给予神经肽不会导致这些标志物的重新表达。