In this work, a simple, reliable, and fast capillary electrophoretic method was developed and validated for the simultaneous determination of 12 polyphenolic compounds, the most frequently found in carob's pulp and seeds. The present work deals with the development of a novel dual electrophoretic system based on the combined use of β-CD and ionic liquid (IL) as buffer additives. A baseline separation of the target analytes was achieved in less than 10 min by using a BGE consisting of 35 mM borate along with 15 mM β-CD and 3 mM l-alanine tert butyl ester lactate (l-AlaC₄Lac) IL as buffer additives at pH 9.5, a temperature of 25°C, and an applied voltage of 30 kV. The application of the developed electrophoretic method to real samples enabled the identification and quantification of the main phenolic constituents of both ripe and unripe carob pulp extracts. The results revealed the predominance of gallic acid in both ripe (183.92 μg/g carob pulp) and unripe (205.10 μg/g carob pulp) carob pulp and highlighted the great influence of the ripening stage on carobs polyphenolic composition, with unripe pods being more enriched in polyphenols (total phenolics detected: 912.58 and 283.13 μg/g unripe and ripe carob pulp).

中文翻译：

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β-环糊精与离子液体联合作为电解液添加剂用于EKC分离测定角豆酚类物质的协同效应研究

在这项工作中，开发并验证了一种简单、可靠、快速的毛细管电泳方法，可同时测定角豆果肉和种子中最常见的 12 种多酚化合物。目前的工作涉及开发一种新型双电泳系统，该系统基于 β-CD 和离子液体 (IL) 作为缓冲添加剂的组合使用。使用由 35 mM 硼酸盐和 15 mM β-CD 和 3 mM L-丙氨酸乳酸叔丁酯 ( L -AlaC ₄Lac) IL 作为缓冲添加剂，pH 9.5，温度 25°C，施加电压 30 kV。将所开发的电泳方法应用于实际样品，可以鉴定和量化成熟和未成熟角豆果肉提取物的主要酚类成分。结果表明，没食子酸在成熟（183.92 μg/g 角豆果肉）和未成熟（205.10 μg/g 角豆果肉）角豆果肉中均占优势，并突出了成熟阶段对角豆树多酚成分的巨大影响，未成熟的豆荚更多富含多酚（检测到的总酚类物质：912.58 和 283.13 μg/g 未成熟和成熟的角豆果肉）。