TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure. We present a TRIzol-modified method of extracting DNA from tissues using the TRIzol reagent and a silica column, which requires only one-third of the time required for the classic extraction procedure. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the DNA extracted using the TRIzol-modified method is ideal and equal to that obtained by the classic method and commercial DNAiso methods. The DNA extracted by the TRIzol-modified method had the same performance in a restriction enzyme digestion and quantitative PCR as that extracted using the classic method. Using the TRIzol-modified method saves time, simplifies the DNA extraction procedure, and facilitates various molecular biology assays.

TRIzol 是苯酚和异硫氰酸胍的单相溶液，用于从组织或细胞中提取 RNA、DNA 和蛋白质。然而，由于其耗时的过程，很少有研究描述其在 DNA 提取中的应用。我们提出了一种使用 TRIzol 试剂和硅胶柱从组织中提取 DNA 的 TRIzol 改进方法，该方法只需要经典提取程序所需时间的三分之一。分光光度分析表明，TRIzol修饰法提取的DNA的260/280和260/230 nm光密度比是理想的，与经典方法和商业DNAiso方法获得的光密度比相当。TRIzol 修饰法提取的 DNA 在限制性内切酶消化和定量 PCR 中与经典方法提取的 DNA 具有相同的性能。