In this work, a novel colorimetric assay based on polythiophene derivative (PMNT) was designed for the detection of protein kinase A (PKA). PKA can catalyze the phosphorylation of peptide, leading to the conformation change of PMNT from random-coil to planar, with the disappearance of absorption peaks above 500 nm and a color change from pink to yellow. The fabricated assay exhibits a wide linear range of 0.05 – 20 U/mL with a detection limit of 0.02 U/mL for PKA activity detection. The proposed protocol has promising prospects for use in clinical diagnosis related to PKA activity.

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中文翻译：

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基于阳离子聚噻吩的比色法检测蛋白激酶 A 的活性

在这项工作中，设计了一种基于聚噻吩衍生物 (PMNT) 的新型比色法，用于检测蛋白激酶 A (PKA)。PKA可以催化肽的磷酸化，导致PMNT的构象从随机螺旋变为平面，500 nm以上吸收峰消失，颜色由粉红色变为黄色。制造的测定显示出 0.05 – 20 U/mL 的宽线性范围，PKA 活性检测的检测限为 0.02 U/mL。所提出的协议在与 PKA 活动相关的临床诊断中具有广阔的前景。

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