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Burkholderia multivorans requires species-specific GltJK for entry of a contact-dependent growth inhibition system protein
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-07-08 , DOI: 10.1111/mmi.14783
Tanya Myers-Morales 1 , Martha M S Sim 1 , Tanner J DuCote 1 , Erin C Garcia 1
Affiliation  

Interbacterial antagonism and communication are driving forces behind microbial community development. In many Gram-negative bacteria, contact-dependent growth inhibition (CDI) systems contribute to these microbial interactions. CDI systems deliver the toxic C-terminus of a large surface exposed protein to the cytoplasm of neighboring bacteria upon cell−contact. Termed the BcpA-CT, import of this toxic effector domain is mediated by specific, yet largely unknown receptors on the recipient cell outer and inner membranes. In this study, we demonstrated that cytoplasmic membrane proteins GltJK, components of a predicted ABC-type transporter, are required for entry of CDI system protein BcpA-2 into Burkholderia multivorans recipient cells. Consistent with current CDI models, gltJK were also required for recipient cell susceptibility to a distinct BcpA-CT that shared sequences within the predicted “translocation domain” of BcpA-2. Strikingly, this translocation domain showed low sequence identity to the analogous region of an Escherichia coli GltJK-utilizing CDI system protein. Our results demonstrated that recipient bacteria expressing E. coli gltJK were resistant to BcpA-2-mediated interbacterial antagonism, suggesting that BcpA-2 specifically recognizes Burkholderia GltJK. Using a series of chimeric proteins, the specificity determinant was mapped to Burkholderia-specific sequences at the GltK C-terminus, providing insight into BcpA transport across the recipient cell cytoplasmic membrane.

中文翻译:

Burkholderia multivorans 需要物种特异性 GltJK 才能进入接触依赖性生长抑制系统蛋白

细菌间的拮抗和交流是微生物群落发展的驱动力。在许多革兰氏阴性细菌中,接触依赖性生长抑制 (CDI) 系统有助于这些微生物相互作用。CDI 系统在细胞接触时将大表面暴露蛋白质的有毒 C 末端传递到邻近细菌的细胞质。被称为 BcpA-CT,这种毒性效应结构域的输入是由受体细胞外膜和内膜上的特定但很大程度上未知的受体介导的。在这项研究中,我们证明了细胞质膜蛋白 GltJK(预测的 ABC 型转运蛋白的成分)是 CDI 系统蛋白 BcpA-2 进入多食伯克霍尔德菌受体细胞所必需的。与当前 CDI 模型一致,gltJK受体细胞对独特的 BcpA-CT 的敏感性也需要,该 BcpA-CT 在 BcpA-2 的预测“易位域”内共享序列。引人注目的是,该易位域与利用 CDI 系统蛋白的大肠杆菌GltJK的类似区域显示出低序列同一性。我们的研究结果表明,表达大肠杆菌gltJK的受体细菌对 BcpA-2 介导的细菌间拮抗作用具有抗性,这表明 BcpA-2 特异性识别伯克霍尔德菌GltJK。使用一系列嵌合蛋白,将特异性决定簇映射到GltK C 末端的伯克霍尔德菌特异性序列,从而深入了解 BcpA 跨受体细胞细胞质膜的转运。
更新日期:2021-07-08
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