A novel Geotrichum candidum aldehyde dehydrogenase (GcALDH) effectively oxidized aldehydes to carboxylic acids under mild conditions. Nonetheless, the free form of GcALDH still had a limitation in stability and recyclability. Herein, to overcome these limitations, this study achieved the immobilization of the GcALDH by the organic-inorganic nanocrystal formation. The GcALDH nanocrystal exhibited 261 ± 40 % of activity comparing to the free enzyme. It also exhibited improved thermostability and recyclability as well as remained excellent substrate promiscuity, which suggested its potential use in green industries. To the best of our knowledge, this is the first time to report the ALDH-inorganic nanocrystal formation with superior properties.

一种新型白地霉醛脱氢酶 ( Gc ALDH) 在温和条件下有效地将醛氧化为羧酸。尽管如此，游离形式的Gc ALDH 在稳定性和可回收性方面仍然存在局限性。在此，为了克服这些限制，本研究通过有机-无机纳米晶体的形成实现了Gc ALDH的固定。该GC与游离酶相比，ALDH 纳米晶体表现出 261±40% 的活性。它还表现出改进的热稳定性和可回收性以及保持优异的基材混杂性，这表明其在绿色工业中的潜在用途。据我们所知，这是首次报道具有优异性能的 ALDH-无机纳米晶形成。