Abstract

The effects of superoxide dismutase (SOD) inhibitors, diethyldithiocarbamate (DDC), triethylenetetramine (trien), and their combination with glucose on cells of the epidermis from pea leaves of different age (rapidly growing young leaves and slowly growing old leaves) was investigated. DDC and trien caused death of the guard cells as determined by destruction of their nuclei. Glucose did not affect destruction of the nuclei induced by SOD inhibitors in the cells from old leaves, but intensified it in the cells from young leaves. 2-Deoxyglucose, an inhibitor of glycolysis, and propyl gallate, SOD-mimic and antioxidant, suppressed destruction of the nuclei that was caused by SOD inhibitors and glucose in cells of the epidermis from the young, but not from the old leaves. Glucose and trien stimulated, and propyl gallate reduced generation of reactive oxygen species (ROS) in the pea epidermis as determined by the fluorescence of 2′,7′-dichlorofluorescein (DCF). Carbonyl cyanide m-chlorophenylhydrazone (CCCP), a protonophoric uncoupler of oxidative and photosynthetic phosphorylation, suppressed the DCF fluorescence in the guard cells. Treatment of the cells with CCCP followed by its removal with washing increased destruction of the nuclei caused by SOD inhibitors and glucose. In young leaves, CCCP was less effective than in old ones. The findings demonstrate the effects of SOD inhibitors and glucose on the cell death and generation of ROS and could indicate glycolysis-dependent ROS production.

中文翻译：

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超氧化物歧化酶抑制剂和葡萄糖对豌豆叶片细胞死亡和活性氧产生的影响

摘要

研究了超氧化物歧化酶 (SOD) 抑制剂、二乙基二硫代氨基甲酸酯 (DDC)、三亚乙基四胺 (trien) 及其与葡萄糖的组合对不同年龄豌豆叶（快速生长的幼叶和缓慢生长的老叶）表皮细胞的影响。DDC 和 trien 导致保卫细胞的死亡，这取决于它们的细胞核的破坏。葡萄糖对老叶细胞中SOD抑制剂诱导的细胞核破坏没有影响，但在幼叶细胞中会加剧这种破坏。2-脱氧葡萄糖（糖酵解抑制剂）和没食子酸丙酯（SOD 模拟物和抗氧化剂）抑制了由 SOD 抑制剂和葡萄糖在幼叶表皮细胞中引起的细胞核破坏，但不能抑制老叶。葡萄糖和trien刺激，根据 2',7'-二氯荧光素 (DCF) 的荧光测定，没食子酸丙酯减少了豌豆表皮中活性氧 (ROS) 的产生。羰基氰间氯苯腙 (CCCP) 是一种氧化和光合磷酸化的质子解偶联剂，可抑制保卫细胞中的 DCF 荧光。用 CCCP 处理细胞，然后通过洗涤去除细胞，增加了由 SOD 抑制剂和葡萄糖引起的细胞核破坏。在幼叶中，CCCP 不如老叶有效。这些发现证明了 SOD 抑制剂和葡萄糖对细胞死亡和 ROS 产生的影响，并可能表明糖酵解依赖的 ROS 产生。