Abstract

The review focuses on the main factors involved in the formation of nonspecific products in isothermal nucleic acid amplification, such as mispriming, ab initio DNA synthesis, and additional activities of DNA polymerases, and discusses approaches to prevent formation of such nonspecific products in LAMP, RPA, NASBA, RCA, SDA, LSDA, NDA, and EXPAR.

中文翻译：

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等温核酸扩增反应中的非特异性合成

摘要

该综述重点关注在等温核酸扩增中形成非特异性产物的主要因素，例如错误引发、从头开始的DNA 合成和 DNA 聚合酶的额外活性，并讨论了防止在 LAMP、RPA 中形成此类非特异性产物的方法、NASBA、RCA、SDA、LSDA、NDA 和 EXPAR。