Abstract

Skeletal muscle also plays a vital role in regulating the movement energy storage and health of metabolism. In order to investigate the expression profile of protein and phosphor-proteins in chicken skeletal muscle during embryonic development, we performed phosphor-proteomics analysis by label-free and TiO₂ enrichment strategy in chicken leg muscle tissues of at embryonic age embryo day 7(E7), E12, E17 and 3-day post-hatch (D3). The study led to the identification of 4332 proteins in the proteome and 1043 phosphorylation modification sites in the phosphorylated proteome, corresponding to 718 proteins (FC ≥ 2 or FC ≤ 0.5 and p < 0.05). The DEP-associated biological processes were involved in Focal adhesion, Glycolysis/gluconeogenesis, Arginine and proline metabolism by KEGG analysis. PPI analyses revealed that these DEPs TNNC1, TNNC2, TNNT2, TNNT3 and phosphorylated DEPs MYLPF interacted with involved pathways. Integrative analysis of proteome and phosphoproteome data found 324 common proteins, corresponding to 521 modification sites and Focal adhesion was the only pathway significantly enriched. These results provide a basis for further understanding the proteome and phosphoproteome and their regulatory biochemical pathways during the development of embryonic chicken skeletal muscle.

摘要

骨骼肌在调节运动能量储存和新陈代谢健康方面也起着至关重要的作用。为了研究胚胎发育过程中鸡骨骼肌中蛋白质和磷蛋白的表达谱，我们通过无标记和 TiO 2_富集策略对胚胎第 7 天（E7）的鸡腿肌肉组织进行了磷蛋白质组学分析)、E12、E17 和孵化后 3 天 (D3)。该研究导致鉴定了蛋白质组中的 4332 个蛋白质和磷酸化蛋白质组中的 1043 个磷酸化修饰位点，对应于 718 个蛋白质（FC ≥ 2 或 FC ≤ 0.5 和p < 0.05)。通过 KEGG 分析，DEP 相关的生物过程涉及黏着斑、糖酵解/糖异生、精氨酸和脯氨酸代谢。PPI 分析表明，这些 DEPs TNNC1、TNNC2、TNNT2、TNNT3 和磷酸化 DEPs MYLPF 与相关通路相互作用。蛋白质组和磷酸化蛋白质组数据的综合分析发现 324 种常见蛋白质，对应于 521 个修饰位点，粘着斑是唯一显着富集的途径。这些结果为进一步了解蛋白质组和磷酸化蛋白质组及其在鸡胚骨骼肌发育过程中的调控生化途径提供了基础。