Journal of Industrial Textiles ( IF 3.2 ) Pub Date : 2021-07-07 , DOI: 10.1177/15280837211027785 Anahita Rohani Shirvan 1 , Nahid Hemmatinejad 1 , S Hajir Bahrami 1 , Azadeh Bashari 1
In the present study a double layer mucoadhesive buccal film containing nanocarriers encapsulated with neem extract was fabricated through electrospinning and solvent casting techniques for dental therapeutic applications. The morphological, physical and mucoadhesive properties of the resulting electrospun and solvent cast oral films were mutually compared, and their drug release behavior and antibacterial activity were further investigated. Chitosan/poly(vinylalcohol) (PVA) as a mucoadhesive component and phenylalanine amino acid nanotubes (PhNTs)-containing neem extract as a drug nanocarrier were used to fabricate oral films. A poly(caprolactone) (PCL) layer was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss. The results indicated an interconnected porous and fully filled solid structures for electrospun and solvent cast films, respectively. The physicomechanical parameters of the samples such as pH, weight, thickness, folding endurance and tensile strength were also evaluated. The crosslinked electrospun buccal film indicated better swelling and mucoadhesive properties compared to the solvent cast film. In addition, the drug loading capacity and encapsulation efficiency of the solvent cast film showed lower experimental values than those of electrospun oral film. On the other hand, the electrospun oral film had a well-controlled release of neem extract up to 82% at oral pH, which is best fitted to the Weibull model, and demonstrated the highest antibacterial properties against S. mutans bacteria with high biocompatibility on L929 fibroblast cells. Generally, the synthesized electrospun mucoadhesive film has a better potential for oral therapeutic applications than the solvent cast film.
中文翻译:
溶剂浇铸法和静电纺丝法制备含楝树提取物的口腔膜的比较
在本研究中,通过静电纺丝和溶剂浇铸技术制造了一种双层粘膜粘附颊膜,其中含有用印楝提取物封装的纳米载体,用于牙科治疗应用。相互比较所得静电纺丝和溶剂流延口腔膜的形态、物理和粘膜粘附特性,并进一步研究它们的药物释放行为和抗菌活性。壳聚糖/聚乙烯醇(PVA)作为粘膜粘附成分,含有苯丙氨酸氨基酸纳米管(PhNTs)的印楝提取物作为药物纳米载体用于制备口腔薄膜。聚(己内酯)(PCL)层用作不可渗透的背衬层,以保护粘膜粘附成分免受舌头运动和药物损失。结果表明电纺膜和溶剂流延膜分别具有相互连接的多孔和完全填充的固体结构。还评估了样品的物理机械参数,例如pH、重量、厚度、耐折性和拉伸强度。与溶剂流延膜相比,交联电纺口腔膜显示出更好的溶胀和粘膜粘附性能。此外,溶剂流延膜的载药量和包封率的实验值低于电纺口腔膜。另一方面,电纺口腔膜在口腔 pH 值下具有高达 82% 的印楝提取物的良好控制释放,最适合 Weibull 模型,并显示出最高的抗菌性能 还评估了样品的物理机械参数,例如pH、重量、厚度、耐折性和拉伸强度。与溶剂流延膜相比,交联电纺口腔膜显示出更好的溶胀和粘膜粘附性能。此外,溶剂流延膜的载药量和包封率的实验值低于电纺口腔膜。另一方面,电纺口腔膜在口腔 pH 值下具有高达 82% 的印楝提取物的良好控制释放,最适合 Weibull 模型,并显示出最高的抗菌性能 还评估了样品的物理机械参数,例如pH、重量、厚度、耐折性和拉伸强度。与溶剂流延膜相比,交联电纺口腔膜显示出更好的溶胀和粘膜粘附性能。此外,溶剂流延膜的载药量和包封率的实验值低于电纺口腔膜。另一方面,电纺口腔膜在口腔 pH 值下具有高达 82% 的印楝提取物的良好控制释放,最适合 Weibull 模型,并显示出最高的抗菌性能 与溶剂流延膜相比,交联电纺口腔膜显示出更好的溶胀和粘膜粘附性能。此外,溶剂流延膜的载药量和包封率的实验值低于电纺口腔膜。另一方面,电纺口腔膜在口腔 pH 值下具有高达 82% 的印楝提取物的良好控制释放,最适合 Weibull 模型,并显示出最高的抗菌性能 与溶剂流延膜相比,交联电纺口腔膜显示出更好的溶胀和粘膜粘附性能。此外,溶剂流延膜的载药量和包封率的实验值低于电纺口腔膜。另一方面,电纺口腔膜在口腔 pH 值下具有高达 82% 的印楝提取物的良好控制释放,最适合 Weibull 模型,并显示出最高的抗菌性能S. mutans细菌对 L929 成纤维细胞具有高生物相容性。通常,合成的电纺粘膜粘附膜比溶剂流延膜具有更好的口腔治疗应用潜力。