In the present study, we examined the impact of three seed priming agents; H₂O₂, GA₃ and NaCl on the oxidative stress status in primed seed germination dynamics and growth establishment of cauliflower seedlings subsequently grown under salt stress. Results showed beneficial effect of all priming agents in improving plant responses to salinity. However, specific differences, depending on development stage, organ and priming type were recorded. At the germination stage, GA₃ was the most advantageous agent to accelerate seedling emergence. At the seedling stage, seedlings obtained from redried H₂O₂-primed seeds showed the highest level of seed superoxide anion (5-fold) and H₂O₂ (4-fold), resulting in higher MDA content (2.4-fold). At the same time, priming seeds with H₂O₂ triggered the overall seedling antioxidant defense, including enzymatic (SOD, CAT, GPX and APX) and non-enzymatic systems (AsA, GSH and proline). Salinity restricted the growth of both leaves (43%) and roots (60%) of cauliflower seedlings issued from unprimed seeds. Under primed conditions, the relative contribution of GA₃ and NaCl was more associated with growth establishment and osmotic adjustment. However, H₂O₂ role was greatly correlated with the antioxidant defense. Indeed, salt-treated seedlings issued from H₂O₂-primed seeds showed the lowest levels of superoxide anion, H₂O₂ and MDA, resulting in the highest activities of the entire antioxidant systems. These findings support the hypothesis that cauliflower plants, when exposed to salt stress benefit from H₂O₂ seed priming, as a pre-germination stimulus, to prevent itself from subsequent oxidative injuries, which supports the hypothesis of “stress memory” of subsequent salt stress.

在本研究中，我们检查了三种种子引发剂的影响；H ₂ O ₂、GA ₃和NaCl对随后在盐胁迫下生长的花椰菜幼苗在引发种子萌发动力学和生长建立中的氧化应激状态的影响。结果显示所有引发剂在改善植物对盐度的响应方面的有益作用。然而，具体的差异取决于发育阶段、器官和启动类型。在萌发阶段，GA ₃是最有利于加速出苗的药剂。在幼苗阶段，从重新干燥的 H ₂ O ₂引发的种子中获得的幼苗显示出最高水平的种子超氧阴离子（5 倍）和 H₂ O ₂（4 倍），导致更高的 MDA 含量（2.4 倍）。同时，用 H ₂ O ₂引发种子引发整体幼苗抗氧化防御，包括酶促（SOD、CAT、GPX 和 APX）和非酶促系统（AsA、GSH 和脯氨酸）。盐度限制了未经处理的种子发出的花椰菜幼苗的叶 (43%) 和根 (60%) 的生长。在引发条件下，GA ₃和NaCl的相对贡献与生长建立和渗透调节更相关。然而，H ₂ O _{2 的}作用与抗氧化防御密切相关。事实上，从 H ₂ O ₂发出的盐处理幼苗处理过的种子显示出最低水平的超氧阴离子、H ₂ O ₂和 MDA，从而导致整个抗氧化系统的活性最高。这些发现支持花椰菜植物在暴露于盐胁迫时受益于 H ₂ O ₂种子启动作为萌发前刺激以防止自身随后发生氧化损伤的假设，这支持了随后盐分“应激记忆”的假设压力。