当前位置: X-MOL 学术Pestic. Biochem. Phys. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bacillus subtilis HSY21 can reduce soybean root rot and inhibit the expression of genes related to the pathogenicity of Fusarium oxysporum
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2021-07-07 , DOI: 10.1016/j.pestbp.2021.104916
Songyang Han 1 , Jiaxin Chen 1 , Yujie Zhao 1 , Hongsheng Cai 1 , Changhong Guo 2
Affiliation  

Soybean root rot occurs globally and seriously affects soybean production. To avoid the many disadvantages of chemical fungicides, the addition of Bacillus is gradually becoming an alternative strategy to tackle soybean root rot. However, the molecular mechanism of phytopathogenic fungi in this process by Bacillus inhibition is rarely reported. In this study, we isolated a strain of B. subtilis HSY21 from soybean rhizosphere soil, which had an inhibition rate of 81.30 ± 0.15% (P < 0.05) against Fusarium oxysporum. The control effects of this strain against soybean root rot under greenhouse and field conditions were 63.83% and 57.07% (P < 0.05), respectively. RNA-seq analysis of F. oxysporum after treatment with strain HSY21 revealed 1445 downregulated genes and 1561 upregulated genes. Among them, genes involved in mycelial growth, metabolism regulation, and disease-related enzymes were mostly downregulated. The activities of cellulase, β-glucosidase, α-amylase, and pectin-methyl- galacturonase as well as levels of oxalic acid and ergosterol in F. oxysporum were significantly decreased after HSY21 treatment. These results demonstrated that B. subtilis HSY21 could effectively control F. oxysporum by inhibiting its growth and the expression of pathogenic genes, thus indicating that this strain may be an ideal candidate for the prevention and control of soybean root rot.



中文翻译:

枯草芽孢杆菌HSY21可减轻大豆根腐病并抑制尖孢镰刀菌致病相关基因的表达

大豆根腐病在全球范围内发生,严重影响大豆生产。为了避免化学杀菌剂的诸多弊端,添加芽孢杆菌正逐渐成为解决大豆根腐病的替代策略。然而,关于芽孢杆菌抑制这一过程中植物病原真菌的分子机制却鲜有报道。在本研究中,我们从大豆根际土壤中分离出了一株枯草HSY21,其 对尖孢镰刀菌的抑制率为 81.30 ± 0.15% ( P < 0.05) 。该菌株在温室和大田条件下对大豆根腐病的防效分别为63.83%和57.07%(P <  0.05)。RNA-seq 分析用菌株 HSY21 处理后的尖孢镰刀菌显示 1445 个下调基因和 1561 个上调基因。其中,涉及菌丝生长、代谢调节和疾病相关酶的基因大多被下调。HSY21处理后F. oxysporum中纤维素酶、β-葡萄糖苷酶、α-淀粉酶和果胶-甲基-半乳糖醛酸酶的活性以及草酸和麦角甾醇的水平显着降低。这些结果表明枯草芽孢杆菌HSY21可以通过抑制尖孢镰刀菌的生长和致病基因的表达来有效地控制尖孢镰刀菌,表明该菌株可能是防治大豆根腐病的理想候选菌株。

更新日期:2021-08-23
down
wechat
bug