当前位置: X-MOL 学术Mol. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Verticillium dahliae secreted protein Vd424Y is required for full virulence, targets the nucleus of plant cells, and induces cell death
Molecular Plant Pathology ( IF 4.9 ) Pub Date : 2021-07-07 , DOI: 10.1111/mpp.13100
Lisen Liu 1, 2 , Zhaohan Wang 1 , Jianing Li 1 , Ye Wang 1 , Jiachen Yuan 1, 3 , Jingjing Zhan 1 , Peng Wang 1 , Yongjun Lin 2 , Fuguang Li 1 , Xiaoyang Ge 1, 3
Affiliation  

Fungal pathogens secrete effector proteins that regulate host immunity and can suppress basal defence mechanisms against colonization in plants. Verticillium dahliae is a widespread and destructive soilborne fungus that can cause vascular wilt disease and reduces plant yields. However, little is currently known about how the effectors secreted by Vdahliae function. In this study, we analysed and identified 34 candidate effectors in the Vdahliae secretome and found that Vd424Y, a glycoside hydrolase family 11 protein, was highly upregulated during the early stages of Vdahliae infection in cotton plants. This protein was located in the nucleus and its deletion compromised the virulence of the fungus. The transient expression of Vd424Y in Nicotiana benthamiana induced BAK1- and SOBIR1-dependent cell death and activated both salicylic acid and jasmonic acid signalling. This enhanced its resistance to the oomycetes Phytophthora capsici in a way that depended on its nuclear localization signal and signal peptides. Our results demonstrate that Vd424Y is an important effector protein targeting the host nucleus to regulate and activate effector-triggered immunity in plants.

中文翻译:

大丽花轮枝菌分泌蛋白 Vd424Y 是完全毒力所必需的,靶向植物细胞核并诱导细胞死亡

真菌病原体分泌调节宿主免疫的效应蛋白,并可以抑制植物中针对定植的基础防御机制。黄萎病菌是一种分布广泛且具有破坏性的土传真菌,可引起血管枯萎病并降低植物产量。然而,目前对V的效应器如何分泌知之甚少。 大丽花功能。在这项研究中,我们分析并确定了V中的 34 个候选效应子。 大丽花分泌组,发现Vd424Y是一种糖苷水解酶家族 11 蛋白,在V的早期阶段高度上调。 大丽花棉花植株感染。这种蛋白质位于细胞核中,它的缺失损害了真菌的毒力。Vd424Y本氏烟草中的瞬时表达诱导BAK1SOBIR1依赖性细胞死亡并激活水杨酸和茉莉酸信号传导。这以取决于其核定位信号和信号肽的方式增强了其对辣椒疫霉菌卵菌的抗性。我们的研究结果表明,Vd424Y 是一种重要的效应蛋白,靶向宿主细胞核以调节和激活植物中效应触发的免疫。
更新日期:2021-08-12
down
wechat
bug