The side effects of chemotherapeutic drugs on healthy organs, as well as cancer cells, are one of the main reasons for the high mortality rates of cancer patients. Controlled drug release studies with biocompatible nanoparticles are becoming increasingly important to prevent drawbacks of traditional chemotherapy treatment. In this study, it is aimed to entrap doxorubicin (DOX) into albumin nanoparticles with a high loading capacity to obtain a long-term release profile. Albumin nanoparticles were prepared through the desolvation method and characterized by transmission electron microscopy. The prepared particles were spherical in shape, having a size between 25 and 100 nm, and the entrapment efficiency was about 87%. The in vitro release profile of DOX-loaded albumin nanoparticles exhibited controlled release of the drug up to 124 days (90.37%). The results showed that DOX was efficiently entrapped into the prepared albumin nanoparticles and the drug was released from the carrier in a controlled manner. Assessment of the cytotoxicity of the obtained nanoparticles on human A549 (human lung adenocarcinoma) and HeLa (human cervix adenocarcinoma) cell lines was carried out through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The cancer cell line cytotoxicity results, consistent with the controlled release profile, evidently showed that DOX-loaded albumin nanoparticles can be a good option in the treatment of cancer.

中文翻译：

---

用于癌症药物递送的白蛋白纳米颗粒

化疗药物对健康器官以及癌细胞的副作用是癌症患者死亡率高的主要原因之一。使用生物相容性纳米颗粒控制药物释放研究对于防止传统化学疗法的缺点变得越来越重要。在这项研究中，目的是将阿霉素 (DOX) 捕获到具有高负载能力的白蛋白纳米颗粒中，以获得长期释放曲线。通过去溶剂化法制备白蛋白纳米颗粒，并通过透射电子显微镜对其进行表征。制备的颗粒呈球形，粒径在25～100 nm之间，包封率约为87%。载有 DOX 的白蛋白纳米颗粒的体外释放曲线显示药物的控释长达 124 天 (90.37%)。结果表明，DOX 被有效地包埋到制备的白蛋白纳米颗粒中，并且药物以受控方式从载体中释放出来。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定法评估所获得的纳米颗粒对人A549（人肺腺癌）和HeLa（人宫颈腺癌）细胞系的细胞毒性. 癌细胞系的细胞毒性结果与控释曲线一致，显然表明负载 DOX 的白蛋白纳米颗粒是治疗癌症的良好选择。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定法评估所获得的纳米颗粒对人A549（人肺腺癌）和HeLa（人宫颈腺癌）细胞系的细胞毒性. 癌细胞系的细胞毒性结果与控释曲线一致，显然表明负载 DOX 的白蛋白纳米颗粒是治疗癌症的一个很好的选择。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定法评估所获得的纳米颗粒对人A549（人肺腺癌）和HeLa（人宫颈腺癌）细胞系的细胞毒性. 癌细胞系的细胞毒性结果与控释曲线一致，显然表明负载 DOX 的白蛋白纳米颗粒是治疗癌症的良好选择。