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QTL mapping of yield-related traits in sesame
Molecular Breeding ( IF 3.1 ) Pub Date : 2021-07-01 , DOI: 10.1007/s11032-021-01236-x
Hongxian Mei 1 , Yanyang Liu 1 , Chengqi Cui 1 , Chengda Hu 2 , Fuxin Xie 3 , Lei Zheng 4 , Zhenwei Du 1 , Ke Wu 1 , Xiaolin Jiang 1 , Yongzhan Zheng 1 , Qingrong Ma 2
Affiliation  

Improving yield is one of the most important targets of sesame breeding. Identifying quantitative trait loci (QTLs) of yield-related traits is a prerequisite for marker-assisted selection (MAS) and QTL/gene cloning. In this study, a BC1 population was developed and genotyped with the specific-locus amplified fragment (SLAF) sequencing technology, and a high-density genetic map was constructed. The map consisted of 13 linkage groups, contained 3528 SLAF markers, and covered a total of 1312.52 cM genetic distance, with an average distance of 0.37 cM between adjacent markers. Based on the map, 46 significant QTLs were identified for seven yield-related traits across three environments. These QTLs distributed on 11 linkage groups, each explaining 2.34–71.41% of the phenotypic variation. Of the QTLs, 23 were stable QTLs that were detected in more than one environment, and 20 were major QTLs that explained more than 10% of the corresponding phenotypic variation in at least one environment. Favorable alleles of 38 QTLs originated from the locally adapted variety, Yuzhi 4; the exotic germplasm line, BS, contributed favorable alleles to only 8 QTLs. The results should provide useful information for future molecular breeding and functional gene cloning.



中文翻译:

芝麻产量相关性状的QTL定位

提高产量是芝麻育种最重要的目标之一。鉴定产量相关性状的数量性状基因座(QTL)是标记辅助选择(MAS)和QTL/基因克隆的先决条件。本研究利用特定位点扩增片段(SLAF)测序技术开发了BC 1群体并进行了基因分型,并构建了高密度遗传图谱。该图谱由13个连锁群组成,包含3528个SLAF标记,总遗传距离为1312.52 cM,相邻标记之间的平均距离为0.37 cM。根据该图谱,在三种环境中鉴定出 7 个产量相关性状的 46 个显着 QTL。这些QTL分布在11个连锁群上,每个连锁群解释2.34-71.41%的表型变异。在这些QTL中,23个是在超过一种环境中检测到的稳定QTL,20个是主要QTL,可以解释至少一种环境中超过10%的相应表型变异。38个QTL的有利等位基因来源于本地适应品种育植4号;外来种质系 BS 仅为 8 个 QTL 贡献了有利的等位基因。研究结果将为未来的分子育种和功能基因克隆提供有用的信息。

更新日期:2021-07-07
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