The effects of halophilic bacteria (Halobacillus sp. and Marinobacter sp.) on pyrite and chalcopyrite surface oxidation in artificial seawater are studied by electrochemical impedance spectroscopy (EIS) in conjunction with X-ray diffraction (XRD) and cyclic voltammetry analysis (CV), in order to explain the influence of these microorganisms on the minerals floatability. EIS analyses on pyrite electrodes suggest that biomaterial from both bacteria adheres to the mineral surface, which is reinforced by CV experiments as capacitive currents are promoted by both bacteria. Additionally, XRD analyses of pyrite samples after immersion in artificial seawater with and without bacteria indicate the formation of hematite on the mineral surface in the presence of Halobacillus sp., which together with the adherence of biomaterial could promote the depression of pyrite during flotation. On the other hand, EIS and CV analyses on chalcopyrite electrodes suggest that the adherence of Halobacillus sp. and Marinobacter sp. to the surface of the mineral have no significant effects on the kinetics of the chalcopyrite oxidation processes. These results together with XRD analyses of the chalcopyrite samples after immersion in artificial seawater with and without bacteria suggest that superficial sulphur might have a stronger influence on chalcopyrite flotability than the presence of bacteria.

嗜盐菌的效果（Halobacillus为属和Marinobacter与X射线衍射（XRD）和循环伏安法分析（CV）一起进行了研究通过电化学阻抗谱（EIS）对黄铁矿和在人工海水中的黄铜矿表面氧化属），以解释这些微生物对矿物可浮性的影响。对黄铁矿电极的 EIS 分析表明，来自两种细菌的​​生物材料都粘附在矿物表面，这通过 CV 实验得到加强，因为两种细菌都促进了电容电流。此外，黄铁矿样品浸入含有和不含细菌的人工海水后的 XRD 分析表明，在盐杆菌存在的情况下，矿物表面形成了赤铁矿sp.，与生物材料的粘附一起可以促进浮选过程中黄铁矿的抑制。另一方面，对黄铜矿电极的 EIS 和 CV 分析表明Halobacillus sp.的粘附。和Marinobacter sp。矿物表面对黄铜矿氧化过程的动力学没有显着影响。这些结果与黄铜矿样品浸入有细菌和无细菌的人造海水后的 XRD 分析表明，表面硫对黄铜矿可浮性的影响可能比细菌的存在更大。