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Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost-derived consortium
Biotechnology and Bioengineering ( IF 3.8 ) Pub Date : 2021-07-07 , DOI: 10.1002/bit.27880
Marco A S Kadowaki 1, 2 , Lorenzo Briganti 1 , Danilo E Evangelista 1, 3 , Alberto Echevarría-Poza 4 , Theodora Tryfona 4 , Vanessa O A Pellegrini 1 , Darlan G Nakayama 1 , Paul Dupree 4 , Igor Polikarpov 1
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The heteropolysaccharide xylan is a valuable source of sustainable chemicals and materials from renewable biomass sources. A complete hydrolysis of this major hemicellulose component requires a diverse set of enzymes including endo-β-1,4-xylanases, β-xylosidases, acetylxylan esterases, α-l-arabinofuranosidases, and α-glucuronidases. Notably, the most studied xylanases from glycoside hydrolase family 11 (GH11) have exclusively been endo-β-1,4- and β-1,3-xylanases. However, a recent analysis of a metatranscriptome library from a microbial lignocellulose community revealed GH11 enzymes capable of releasing solely xylobiose from xylan. Although initial biochemical studies clearly indicated their xylobiohydrolase mode of action, the structural features that drive this new activity still remained unclear. It was also not clear whether the enzymes acted on the reducing or nonreducing end of the substrate. Here, we solved the crystal structure of MetXyn11 in the apo and xylobiose-bound forms. The structure of MetXyn11 revealed the molecular features that explain the observed pattern on xylooligosaccharides released by this nonreducing end xylobiohydrolase.

中文翻译:

解锁在堆肥衍生的财团中鉴定的不寻常 GH11 成员的木糖二糖水解酶活性的结构特征

杂多糖木聚糖是来自可再生生物质资源的可持续化学品和材料的宝贵来源。这种主要半纤维素成分的完全水解需要多种酶,包括内切-β-1,4-木聚糖酶、β-木糖苷酶、乙酰木聚糖酯酶、α -l-阿拉伯呋喃糖苷酶和α-葡糖苷酸酶。值得注意的是,研究最多的来自糖苷水解酶家族 11 (GH11) 的木聚糖酶完全是内切-β-1,4- 和 β-1,3-木聚糖酶。然而,最近对来自微生物木质纤维素群落的宏转录组文库的分析揭示了 GH11 酶能够从木聚糖中仅释放木二糖。尽管最初的生化研究清楚地表明了它们的木糖水解酶作用方式,但驱动这种新活性的结构特征仍不清楚。也不清楚酶是作用于底物的还原端还是非还原端。在这里,我们解决了 apo 和木二糖结合形式的 MetXyn11 的晶体结构。
更新日期:2021-09-12
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