Chemical pesticides often applied to effectively control the long-horned beetles to protect the forests are reported to affect the non-target organisms adversely. Dastarcus helophoroides is an active natural enemy of long-horned beetles. Studying the molecular mechanism of P450 genes will help to elucidate the metabolic mechanism of pesticides in D. helophoroides to better coordinate the use of chemical and biological controls. In this study, two novel genes, CYP6BQ21, and CYP6BQ22 were successfully cloned from D. helophoroides using the rapid amplification of cDNA ends technique. The sequence and homology analyses indicated that CYP6BQ21 was highly similar to CYP6BQ1 from Tribolium castaneum, while CYP6BQ22 was closely related to CYP6BQ13 from T. castaneum. Gene expression patterns showed that CYP6BQ21 and CYP6BQ22 were specifically expressed in the adult stage of D. helophoroides. In addition, CYP6BQ21 and CYP6BQ22 were significantly expressed under the treatment of a high concentration of cypermethrin. Based on these findings, we proposed that CYP6BQ21 and CYP6BQ22 played possible roles in the development and pesticide stress of D. helophoroides. Our findings are an important first step in identifying and characterizing CYP6BQ21 and CYP6BQ22 from D. helophoroides, and lay the groundwork for future research into the role of these novel CYP6s in the regulation of pesticide resistance in D. helophoroides.

据报道，经常用于有效控制长角甲虫以保护森林的化学农药会对非目标生物产生不利影响。Dastarcus helophoroides是长角甲虫的活跃天敌。研究P450基因的分子机制将有助于阐明D. helophoroides中农药的代谢机制，以更好地协调化学和生物防治的使用。在这项研究中，使用 cDNA 末端快速扩增技术从D. helophoroides中成功克隆了两个新基因CYP6BQ21和CYP6BQ22。序列和同源性分析表明，CYP6BQ21 与 CYP6BQ1 高度相似，来自Tribolium castaneum，而CYP6BQ22 与来自T. castaneum 的CYP6BQ13 密切相关。基因表达模式表明，CYP6BQ21和CYP6BQ22在D. helophoroides的成虫阶段特异性表达。此外，CYP6BQ21和CYP6BQ22在高浓度氯氰菊酯处理下显着表达。基于这些发现，我们提出CYP6BQ21和CYP6BQ22可能在D. helophoroides的发育和农药胁迫中发挥作用。我们的发现是识别和表征CYP6BQ21和CYP6BQ22从D.花绒寄甲，打下基础为今后的研究这些新CYP6s的抗药性在调控中的作用D.花绒寄甲。