Genotyping of lentil plant genetic resources holds the promise to increase the identification and utilization of useful genetic diversity for crop improvement. The International Center for Agriculture Research in the Dry Areas (ICARDA) lentil reference set plus collection of 176 accessions was genotyped using genotyping-by-sequencing (GBS) and 22,555 SNPs were identified. The population structure was investigated using Bayesian analysis (STRUCTURE, k = 3) and principal component analysis. The two methods are in concordance. Genome-wide association analysis (GWAS) using the filtered SNP set and ICARDA historical phenotypic data discovered putative markers for several agronomic traits including days to first flower, seeds per pod, seed weight and days to maturity. The genetic and genomic resources developed and utilized in this study are available to the research community interested in exploring the ICARDA reference set plus collection using GWAS.

中文翻译：

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使用 ICARDA 扁豆 (Lens culinaris Medik.) Reference Plus 收集的农艺性状的遗传多样性和 GWAS

扁豆植物遗传资源的基因分型有望增加对作物改良有用遗传多样性的识别和利用。国际干旱地区农业研究中心 (ICARDA) 小扁豆参考集加上 176 个种质的集合使用基因分型测序 (GBS) 进行了基因分型，并确定了 22,555 个 SNP。使用贝叶斯分析（STRUCTURE，ķ= 3) 和主成分分析。这两种方法是一致的。使用过滤的 SNP 集和 ICARDA 历史表型数据的全基因组关联分析 (GWAS) 发现了几种农艺性状的推定标记，包括第一次开花的天数、每豆荚的种子、种子重量和成熟天数。本研究中开发和利用的遗传和基因组资源可供有兴趣探索 ICARDA 参考集以及使用 GWAS 收集的研究界使用。