Deficiency of trace metals exacerbates instability in the methanogenic phase of two-phase thermophilic AD. The addition of a micronutrient supplement containing calcium, magnesium, cobalt and nickel was found to help process recovery, increasing methane production by up to 40%. In this study, next gen sequencing was used to identify the changes brought by addition of the micronutrients in the bacterial community of the methanogenic phase of food waste processing. The diversity of the community before supplementation was considerably low and a single species of Phylum Thermotogae was the sole dominant bacterial group. The addition of a micronutrient supplement comprising of Ca, Mg, Co and Ni caused a potent increase in the diversity of the community and species belonging to Arcobacter, Clostridium, Pseudomonas, Bacteroides and Coprothermobacter were particularly enriched. This suggested that the action of the micronutrients resulted in an increase in the functional diversity and redundancy of the bacterial community and limiting hydrogenotrophic methanogenesis in favour of acetotrophic methanogenesis. These factors would contribute to the observed increased stability and higher productivity in the micronutrient supplemented thermophilic AD process.

微量金属的缺乏加剧了两相嗜热 AD 产甲烷阶段的不稳定性。研究发现，添加含有钙、镁、钴和镍的微量营养素补充剂有助于工艺回收，将甲烷产量提高多达 40%。在这项研究中，下一代测序被用来识别添加微量营养素给食物垃圾处理产甲烷阶段的细菌群落带来的变化。补充前群落的多样性相当低，一种单一的嗜热门菌是唯一的优势菌群。添加由 Ca、Mg、Co 和 Ni 组成的微量营养素补充剂可有效增加属于 Arcobacter、梭状芽孢杆菌、假单胞菌、拟杆菌和粪嗜热菌特别丰富。这表明微量营养素的作用导致细菌群落的功能多样性和冗余增加，并限制了氢营养型产甲烷，有利于乙酸营养型产甲烷。这些因素将有助于在补充微量营养素的嗜热 AD 过程中观察到增加的稳定性和更高的生产力。