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Allantoin, a purine metabolite, enhances peripheral nerve regeneration following sciatic nerve injury in rats: A stereological and immunohistochemical study
Journal of Chemical Neuroanatomy ( IF 2.8 ) Pub Date : 2021-07-06 , DOI: 10.1016/j.jchemneu.2021.102002
Burcu Delibas 1 , Enis Kuruoglu 2 , Mehmet Cihan Bereket 3 , Mehmet Emin Onger 4
Affiliation  

Aim

Following peripheral nerve injury, in addition to axonal and myelin degeneration, a sharp increase is observed in cell numbers, especially Schwann cells, in the distal part of the injury. This study investigated the effect of allantoin, involved in purine catabolism, on the reactions occurring in the lesion area.

Material and Method

An experimental sciatic nerve injury model was established with the application of pressure at 50 Newtons for 5 s to the right sciatic nerves of experimental animals following visualization with the help of pliers. Allantoin was administered to the test groups via the intraperitoneal (i.p.) route (10 mg/kg), at the same time every day for 30 days. The animals were sacrificed at the end of 30 days, following electromyography and Sciatic Function Index tests. Myelinated/unmyelinated axon numbers were evaluated stereologically. Myelin sheath thickness, axon diameter, mitotic activity, and functional improvement in muscles in this peripheral nerve degeneration model were investigated. The test results were then subjected to statistical analysis.

Results

Allantoin was observed to exhibit curative effects in terms of function, although stereological tests revealed no morphological differences.

Conclusion

The i.p. administration of allantoin may have a beneficial effect on nerve healing.



中文翻译:

尿囊素是一种嘌呤代谢物,可增强大鼠坐骨神经损伤后的周围神经再生:一项体视学和免疫组织化学研究

目的

周围神经损伤后,除了轴突和髓鞘变性外,还观察到损伤远端的细胞数量,尤其是雪旺细胞数量急剧增加。本研究调查了参与嘌呤分解代谢的尿囊素对病变区域发生的反应的影响。

材料和方法

建立实验性坐骨神经损伤模型,在钳子的帮助下对实验动物右侧坐骨神经施加 50 牛顿的压力,持续 5 秒。通过腹膜内 (ip) 途径 (10 mg/kg) 将尿囊素施用于测试组,每天同一时间,持续 30 天。在肌电图和坐骨神经功能指数测试后,在 30 天结束时处死动物。对有髓/无髓轴突数进行立体学评估。研究了该周围神经变性模型中的髓鞘厚度、轴突直径、有丝分裂活性和肌肉功能改善。然后对测试结果进行统计分析。

结果

观察到尿囊素在功能方面表现出疗效,尽管体视学测试显示没有形态学差异。

结论

腹腔内给予尿囊素可能对神经愈合有益。

更新日期:2021-07-09
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