The use of matrix laser desorption/ionization mass spectrometry (MALDI) for the study of small molecules is still critical and limited by the efficiency of the matrices. In this work, for the first time, the effect of anion and cation in the structure of ionic liquids to obtain high-intensity MALDI spectra of amino acids is shown. Ten ionic liquids based on substituted imidazole were tested. Three amino acids (histidine, alanine and serine) were used as test compounds to assess the effectiveness of ionic liquids. It was found that the BMIMC₆F₅BF₃ ionic liquid, used for the first time as a MALDI matrix, showed the best characteristics in terms of ionization efficiency for the analytes under consideration. Mass spectrometric imaging was used to estimate the degree of homogeneity in the distribution of the analyte over the surface and in the volume of the matrix with ionic liquid. It has been shown that, without ionic liquid, approximately 40% of the total surface area with an extremely low amino acid content is observed on the matrix surface. Whereas in the presence of ionic liquid there are only 3% of such areas. The use of an ionic liquid allows the analyte to be concentrated in the surface layer of the matrix. At the same time, without IL, only when using a classical matrix, there is an uneven distribution of amino acid both in the surface layer and throughout the entire volume of the matrix.

使用基质激光解吸/电离质谱 (MALDI) 研究小分子仍然很关键，并且受到基质效率的限制。在这项工作中，首次展示了阴离子和阳离子在离子液体结构中的作用，以获得氨基酸的高强度 MALDI 光谱。测试了十种基于取代咪唑的离子液体。三种氨基酸（组氨酸、丙氨酸和丝氨酸）用作测试化合物来评估离子液体的有效性。发现 BMIMC ₆ F ₅ BF ₃首次用作 MALDI 基质的离子液体在所考虑的分析物的电离效率方面表现出最佳特性。质谱成像用于估计分析物在表面上和在具有离子液体的基质体积中分布的均匀度。已经表明，在没有离子液体的情况下，在基质表面上观察到大约 40% 的总表面积具有极低的氨基酸含量。而在离子液体存在的情况下，此类区域仅占 3%。使用离子液体可使分析物集中在基质的表层。同时，没有IL，只有在使用经典矩阵时，