The emergence of an inflammatory condition such as asthma could affect the therapeutic potential of stem cells. Synopsis of previous documents yielded controversial outcomes, leading to a limitation of stem cell-based therapy in the clinical setting. This study aimed to assess the impact of asthmatic serum on the MSCs aging and dynamic growth in vitro. Rats were divided into control and asthmatic groups randomly. The asthmatic change was induced using OVA sensitization. The asthmatic structural changes are monitored by conventional Haematoxylin-Eosin staining. Thereafter, blood samples were taken and sera provided from each group. In this study, primary bone marrow mesenchymal stem cells were cultured in culture medium supplemented with normal and asthmatic serum for 7 days. The MSCs viability was examined using the MTT assay. The expression of the aging-related gene (β-galactosidase), and stemness-related markers such as Sox2, Kfl-4 and p16^INK4a were analysed by real-time PCR assay. Histological examination revealed chronic inflammatory remodelling which is identical to asthmatic changes. MTT assay showed a reduction of mesenchymal stem cell viability compared to the control group (P < .05). Real-time PCR analysis revealed a down-regulation of stemness-related markers Sox2, Kfl-4 and p16^INK4a coincided with aging changes (β-galactosidase) compared to the control group (P < .05). These data show the detrimental effect of asthmatic condition on bone marrow regenerative potential by accelerating early-stage aging in different stem cells and further progenitor cell depletion.

中文翻译：

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慢性哮喘病调节骨髓间充质干细胞衰老的发生

哮喘等炎症性疾病的出现可能会影响干细胞的治疗潜力。先前文件的概要产生了有争议的结果，导致基于干细胞的治疗在临床环境中受到限制。本研究旨在评估哮喘血清对体外 MSCs 老化和动态生长的影响。将大鼠随机分为对照组和哮喘组。使用OVA致敏诱导哮喘变化。通过常规苏木精-伊红染色监测哮喘结构变化。此后，从每组采集血液样本并提供血清。在这项研究中，原代骨髓间充质干细胞在补充有正常和哮喘血清的培养基中培养 7 天。使用 MTT 测定检查 MSCs 的活力。β-半乳糖苷酶）和干性相关标志物（如Sox2、Kfl-4和p16 ^INK4a）通过实时 PCR 测定进行分析。组织学检查显示慢性炎症重塑，这与哮喘变化相同。MTT 测定显示与对照组相比，间充质干细胞活力降低 ( P  < .05)。实时 PCR 分析显示，与对照组相比，干细胞相关标志物Sox2、Kfl-4和p16 ^{INK4a 的}下调与衰老变化（β-半乳糖苷酶）一致（P < .05）。这些数据表明，哮喘病通过加速不同干细胞的早期衰老和进一步消耗祖细胞对骨髓再生潜力产生不利影响。