当前位置: X-MOL 学术Biotechnol. Lett. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Metabolic pathway analysis of walnut endophytic bacterium Bacillus subtilis HB1310 related to lipid production from fermentation of cotton stalk hydrolysate based on genome sequencing
Biotechnology Letters ( IF 2.7 ) Pub Date : 2021-07-06 , DOI: 10.1007/s10529-021-03160-8
Qin Zhang 1 , Panpan Liu 2 , Yanbin Li 1 , Hui Jiang 2
Affiliation  

Objectives

In this study, genome sequencing and metabolic analysis were used to identify and verify the key metabolic pathways for glucose and xylose utilization and fatty acid synthesis in the walnut endophytic bacterium (WEB) Bacillus subtilis HB1310.

Results

The genome sequence of WEB HB1310 was generated with a size of 4.1 Mb and GC content of 43.5%. Genome annotation indicated that the Embden–Meyerhof–Parnas, pentose phosphate, and fatty acid synthesis pathways were mainly involved in mixed sugar utilization and lipid production. In particular, diverse and abundant fatty acid synthesis genes were observed in a higher number than in other Bacillus strains. The tricarboxylic acid cycle competitively shared the carbon flux flowing before 48 h, and the acetic acid fermentation competed after 72 h. Moreover, fatty acid synthase activity was highly correlated with lipid titer with a correlation coefficient of 0.9626, and NADPH might be more utilized for the lipid synthesis within 48 h.

Conclusions

This study is the first attempt to explain the metabolic mechanism of mixed sugar utilization and lipid production based on genomic information, which provides a theoretical basis for the metabolic regulation of bacterial lipid production from lignocellulosic hydrolysates.



中文翻译:

基于基因组测序的核桃内生菌Bacillus subtilis HB1310与棉秆水解物发酵产脂质相关的代谢途径分析

目标

在这项研究中,基因组测序和代谢分析被用来识别和验证核桃内生细菌(WEB)枯草芽孢杆菌HB1310中葡萄糖和木糖利用和脂肪酸合成的关键代谢途径。

结果

WEB HB1310的基因组序列大小为4.1 Mb,GC含量为43.5%。基因组注释表明,Embden-Meyerhof-Parnas、戊糖磷酸和脂肪酸合成途径主要参与混合糖利用和脂质生产。特别是,与其他芽孢杆菌菌株相比,观察到的多样化和丰富的脂肪酸合成基因数量更多。三羧酸循环在 48 h 前竞争共享碳通量,72 h 后乙酸发酵竞争。此外,脂肪酸合酶活性与脂质滴度高度相关,相关系数为0.9626,NADPH可能在48小时内更多地用于脂质合成。

结论

本研究首次尝试基于基因组信息解释混合糖利用和脂质生产的代谢机制,为木质纤维素水解产物中细菌脂质生产的代谢调控提供了理论依据。

更新日期:2021-07-06
down
wechat
bug