No fertilized soils (unfertilized and fallow treatments) and soils subjected to 28-year fertilization regimes, including mineral fertilization (PK and NPK) and manure amendments (NPKM and M), were incubated with or without ¹³C - glucose. Results showed that compared to mineral fertilization (0.64 − 0.69), the manure amendments significantly increased microbial C use efficiency (CUE) (0.76 − 0.79), mainly due to higher soil pH, lower resource stoichiometric ratios of dissolved organic C (DOC): mineral N, DOC: available P and mineral N: available P, and lower specific activities (per microbial biomass C unit) of β-1,4-glucosidase, N-acetyl-glucosaminidase and acid phosphatase. Glucose addition increased SOC mineralization, inducing positive priming effect (PE) with lower values in the manure amendments (0.11 − 0.12 mg C g⁻¹ SOC) relative to mineral fertilization (0.25 − 0.55 mg C g⁻¹ SOC). The PE was negatively correlated with soil pH and positively associated with resource stoichiometric ratios of DOC: mineral N, DOC: available P and mineral N: available P, mainly due to microbes mineralizing SOM to release nutrients, as indicated by the positive relationships between PE and the specific activities of N-acetyl-glucosaminidase and acid phosphatase. Concluding, relative to mineral fertilization, long-term manure amendment could increase CUE and decrease PE via alleviated soil acidification and resource limitation, thus facilitating soil C sequestration.

未施肥的土壤（未施肥和休耕处理）和经历 28 年施肥制度的土壤，包括矿物施肥（PK 和 NPK）和肥料改良剂（NPKM 和 M），在有或没有¹³C-葡萄糖。结果表明，与矿物施肥 (0.64 − 0.69) 相比，粪肥改良剂显着提高了微生物 C 的利用效率 (CUE) (0.76 − 0.79)，主要是由于较高的土壤 pH 值、较低的溶解有机 C (DOC) 资源化学计量比：矿物质 N，DOC：有效磷和矿物质 N：有效磷，以及较低的 β-1,4-葡萄糖苷酶、N-乙酰氨基葡萄糖苷酶和酸性磷酸酶的比活性（每微生物生物量 C 单位）。葡萄糖添加增加了 SOC 矿化，诱导了正启动效应 (PE)，相对于矿物施肥 (0.25 - 0.55 mg C g ^-1 )，粪便改良剂 (0.11 - 0.12 mg C g ^-1 SOC) 值较低SOC)。PE与土壤pH呈负相关，与DOC：矿物N、DOC：有效磷和矿物N：有效P的资源化学计量比呈正相关，主要是由于微生物矿化SOM释放养分，PE之间的正相关关系表明以及N-乙酰氨基葡萄糖苷酶和酸性磷酸酶的比活性。最后，相对于矿物施肥，长期粪肥修正案可以通过减轻土壤acidifi增加CUE和减小PE Ç通货膨胀和资源限制，从而有利于土壤碳封存。