Yellow mosaic virus (YMV) transmitted by whitefly (Bemisia tabaci) causes yellow mosaic disease (YMD) in blackgram cultivars only during kharif season but disease was not observed in the same cultivars sown during spring season. The main objective of the present investigation was to study the cooperative role of various antioxidative enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPOX), glutathione reductase (GR), monodehydro ascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) along with non- enzymic antioxidants and free radical scavenging activities under natural epiphytotic disease conditions in black gram cultivars during kharif and spring season. Three black gram cultivars-KUG253 (highly susceptible to YMD), MASH114 (resistant) and Cross (KUG253 × MASH114) at F5 generation were sown during both the seasons. During the kharif season, YMV caused severe chlorosis only in KUG253 cultivar at 40 days after sowing (DAS) which affected its quantum efficiency by 58% as compared to the spring season. Higher specific activities of MDHAR, DHAR, and GR in Cross and MASH114 cultivars during kharif season at 20DAS might have helped these cultivars to fight off YMV right from the beginning of the infection. Cross cultivar showed the highest superoxide anion scavenging activity (SASA), ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity and total reducing power (TRP). On the other hand, KUG253 cultivar struggled to maintain the specific activity of CAT, GR, and DHAR as the disease progressed further. GPOX and APX enzymes might have tried to compensate for the loss of CAT enzyme but high specific activity of NADPH Oxidase promoted the production of harmful ROS which ultimately lead to higher oxidative damage in KUG253 cultivar.

由粉虱（烟粉虱）传播的黄色花叶病毒 (YMV)仅在kharif季节在 blackgram 栽培品种中引起黄色花叶病 (YMD)，但在春季播种的相同栽培品种中未观察到该病。本研究的主要目的是研究各种抗氧化酶的协同作用，如超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、抗坏血酸过氧化物酶 (APX)、愈创木酚过氧化物酶 (GPOX)、谷胱甘肽还原酶 (GR)、单脱氢抗坏血酸还原酶（MDHAR），脱氢叶酸还原酶（DHAR），在期间黑色克品种自然epiphytotic疾病的条件下非酶促抗氧化剂和自由基清除活性沿着夏播和春季。在两个季节都播种了 F5 代的三个黑革兰品种 KUG253（对 YMD 高度敏感）、MASH114（抗性）和 Cross（KUG253 × MASH114）。在秋收季节，YMV只在40日龄KUG253品种播种后（DAS），这相比于春暖花开的季节了58％影响了其量子效率造成了严重的萎黄。在Cross和MASH114品种中MDHAR，DHAR，GR和更高具体活动秋收20DAS 的季节可能帮助这些品种从感染开始就对抗 YMV。杂交品种表现出最高的超氧阴离子清除活性（SASA）、铁还原抗氧化能力（FRAP）、2,2-二苯基-1-苦基肼（DPPH）自由基清除活性和总还原能力（TRP）。另一方面，随着疾病的进一步发展，KUG253 品种努力维持 CAT、GR 和 DHAR 的比活性。GPOX 和 APX 酶可能试图补偿 CAT 酶的损失，但 NADPH 氧化酶的高比活性促进了有害 ROS 的产生，最终导致 KUG253 栽培品种中更高的氧化损伤。