Bacterial vaginosis (BV) is a gynecologic disorder characterized by a shift in cervicovaginal microbiota from Lactobacillus spp. dominance to a polymicrobial biofilm composed of diverse anaerobes. We utilized a well-characterized human three-dimensional cervical epithelial cell model in conjunction with untargeted metabolomics and immunoproteomics analyses to determine the immunometabolic contribution of three members of the Veillonellaceae family: Veillonella atypica, Veillonella montpellierensis and Megasphaera micronuciformis at this site. We found that Veillonella spp. infections induced significant elevation of polyamines. M. micronuciformis infections significantly increased soluble inflammatory mediators, induced moderate levels of cell cytotoxicity, and accumulation of cell membrane lipids relative to Veillonella spp. Notably, both V. atypica and V. montpellierensis infections resulted in consumption of lactate, a key metabolite linked to gynecologic and reproductive health. Collectively our approach and data provide unique insights into the specific contributions of Veillonellaceae members to the pathogenesis of BV and women’s health.

细菌性阴道病 (BV) 是一种妇科疾病，其特征是乳杆菌属的宫颈阴道微生物群发生转变。由多种厌氧菌组成的多微生物生物膜的优势。我们利用表征良好的人类三维宫颈上皮细胞模型结合非靶向代谢组学和免疫蛋白质组学分析来确定Veillonellaceae家族的三个成员的免疫代谢贡献：Veillonella atypica、Veillonella montpellierensis和Megasphaera micronuciformis。我们发现Veillonella spp。感染引起多胺显着升高。微核菌感染显着增加可溶性炎症介质，诱导中等水平的细胞毒性，以及相对于Veillonella spp 的细胞膜脂质积累。值得注意的是，V. atypica和V. montpellierensis感染导致乳酸的消耗，乳酸是与妇科和生殖健康相关的关键代谢物。总的来说，我们的方法和数据为了解Veillonellaceae成员对 BV 发病机制和女性健康的具体贡献提供了独特的见解。