Abstract

Coke-oven workers are occupationally exposed to high concentrations of polycyclic aromatic hydrocarbons. In this study, 1-hydroxypyrene, 9-hydroxyphenantrene, and 3-hydroxybenzo[a]pyrene levels were determined in the urine of occupationally exposed coke-oven worker as markers of exposure to polycyclic aromatic hydrocarbons. The validated high-pressure liquid chromatography method with fluorescence detection was performed for metabolite analysis. Average values of 1-hydroxypyrene were 1.26 µmol/mol creatinine and of 9-hydroxyphenantrene 3.68 µmol/mol creatinine in the workers group, and 0.04 and 0.06 µmol/mol creatinine in the control group. While the average value of 3-hydroxybenzo[a]pyrene was 0.007 µmol/mol creatinine in the workers group, it was below the limit of detection in the control group. All of the mean 1-hydroxypyrene values (coke-oven workers overall mean = 1.26 μmol/mol creatinine; high exposure group mean = 1.91 μmol/mol creatinine; low exposure group mean = 0.66 μmol/mol creatinine) obtained in the workers group were higher than the biological exposure index (0.5 μmol/mol creatinine) that warrants occupational exposure proposed by American Conference of Government Industrial Hygienists. Urinary 1-hydroxypyrene mean levels of all coke-oven workers and the high exposure group exceeded the no observed genotoxic effect level of 1 μmol/mol creatinine.

摘要

焦炉工人职业暴露于高浓度的多环芳烃。在这项研究中，测定了职业暴露焦炉工人尿液中的 1-羟基芘、9-羟基菲和 3-羟基苯并 [a] 芘水平，作为暴露于多环芳烃的标志物。使用经验证的带荧光检测的高压液相色谱法进行代谢物分析。工人组 1-羟基芘的平均值为 1.26 μmol/mol 肌酐，9-羟基菲的平均值为 3.68 μmol/mol 肌酐，对照组为 0.04 和 0.06 μmol/mol 肌酐。工人组3-羟基苯并[a]芘的平均值为0.007 μmol/mol肌酐，低于对照组的检测限。在工人组中获得的所有平均 1-羟基芘值（焦炉工人总体平均值 = 1.26 μmol/mol 肌酐；高暴露组平均值 = 1.91 μmol/mol 肌酐；低暴露组平均值 = 0.66 μmol/mol 肌酐）高于美国政府工业卫生学家会议提出的保证职业暴露的生物暴露指数（0.5 μmol/mol 肌酐）。所有焦炉工人和高暴露组的尿液 1-羟基芘平均水平超过了未观察到的 1 μmol/mol 肌酐的遗传毒性效应水平。5 μmol/mol 肌酐），保证美国政府工业卫生学家会议提出的职业暴露。所有焦炉工人和高暴露组的尿液 1-羟基芘平均水平超过了未观察到的 1 μmol/mol 肌酐的遗传毒性效应水平。5 μmol/mol 肌酐），保证美国政府工业卫生学家会议提出的职业暴露。所有焦炉工人和高暴露组的尿液 1-羟基芘平均水平超过了未观察到的 1 μmol/mol 肌酐的遗传毒性效应水平。