The present work demonstrates the synthesis of kaolin supported Ag nanoparticles (AgNPs@Kaolin) by using an aqueous extract of Thymbra spicata as a green reductant and capping agent. Physicochemical characteristics of the synthesized nanocomposite were studied by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), elemental mapping, X-ray diffraction (XRD) and inductively coupled plasma (ICP) techniques. The biogenic AgNPs are uniformly globular. Owing to the surface modification by the plant derived phytochemicals, the NPs are found to be distributed evenly on the kaolin surface. The AgNPs@Kaolin nanocomposite has been explored biologically in the anticancer and antioxidant assays. In the cellular and molecular part of the recent study, the treated cells with AgNPs@Kaolin nanocomposite were assessed by MTT assay for 48 h about the cytotoxicity and anti-human lung adenocarcinoma properties on normal (HUVEC) and lung adenocarcinoma cell lines i.e. lung well-differentiated bronchogenic adenocarcinoma (HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated adenocarcinoma (PC-14). The viability of malignant lung cell line reduced dose-dependently in the presence of AgNPs@Kaolin nanocomposite. The IC50 of AgNPs@Kaolin nanocomposite were 509, 315, and 189 µg/mL against HLC-1, LC-2/ad, and PC-14 cell lines, respectively. In the antioxidant test, the IC50 of AgNPs@Kaolin nanocomposite and BHT against DPPH free radicals were 125 and 161 µg/mL, respectively. After the clinical study, AgNPs@Kaolin nanocomposite containing T. spicata leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human lung adenocarcinoma.

目前的工作证明了通过使用Thymbra spicata的水提取物作为绿色还原剂和封端剂来合成高岭土负载的银纳米粒子 (AgNPs@Kaolin) 。通过研究合成的纳米复合材料的理化特性 场发射扫描电子显微镜 (FESEM)、透射电子显微镜 (TEM)、能量色散 X 射线光谱 (EDS)、元素映射、X 射线衍射 (XRD) 和电感耦合等离子体 (ICP) 技术。生物 AgNPs 是均匀的球状。由于植物来源的植物化学物质的表面改性，发现纳米颗粒均匀分布在高岭土表面。AgNPs@Kaolin 纳米复合材料已在抗癌和抗氧化试验中进行了生物学研究。在最近研究的细胞和分子部分，通过 MTT 法对用 AgNPs@Kaolin 纳米复合材料处理的细胞进行 48 小时评估，评估其对正常（HUVEC）和肺腺癌细胞系（即肺孔）的细胞毒性和抗人肺腺癌特性。 -分化的支气管腺癌（HLC-1），肺中分化腺癌（LC-2/ad）和肺低分化腺癌（PC-14）。在 AgNPs@Kaolin 纳米复合材料的存在下，恶性肺细胞系的活力呈剂量依赖性降低。AgNPs@Kaolin 纳米复合材料对 HLC-1、LC-2/ad 和 PC-14 细胞系的 IC50 分别为 509、315 和 189 µg/mL。在抗氧化测试中，AgNPs@Kaolin 纳米复合材料和 BHT 对 DPPH 自由基的 IC50 分别为 125 和 161 µg/mL。经过临床研究，AgNPs@Kaolin 纳米复合材料含有 和 PC-14 细胞系。在抗氧化测试中，AgNPs@Kaolin 纳米复合材料和 BHT 对 DPPH 自由基的 IC50 分别为 125 和 161 µg/mL。经过临床研究，AgNPs@Kaolin 纳米复合材料含有 和 PC-14 细胞系。在抗氧化测试中，AgNPs@Kaolin 纳米复合材料和 BHT 对 DPPH 自由基的 IC50 分别为 125 和 161 µg/mL。经过临床研究，AgNPs@Kaolin 纳米复合材料含有虎尾草叶水提物可用于配制新的化疗药物或补充剂，以治疗多种类型的人肺腺癌。