Seven metal-resistant yeast strains were isolated and selected from Dayet Oum Ghellaz Lake water (northwest of Algeria) known as a highly polluted area by lead and cadmium. The yeast strains were screened on the basis of their resistance to seven heavy metals Hg, Cr, Cd, Pb, Cu, Zn, and Fe and characterized by molecular and phylogenetic analysis. The sequencing of the D1/D2 domain of the 26S rRNA genes revealed the affiliation of the seven yeast isolates to Rhodotorula mucilaginosa, Clavispora lusitaniae, and Wickerhamomyces anomalus species. All yeast strains were halotolerant as they were able to grow in 10–15% NaCl. The yeast isolates were highly resistant to the studied heavy metals and exhibited different tolerance according to the metal type. The highest minimum inhibitory concentrations (MIC) were observed in R. mucilaginosa RO7 and W. anomalus WO2 strains which were then selected for lead removal assays. The present study is the first to investigate the lead elimination by W. anomalus. The lead uptake was significantly affected by biomass concentration in a reverse relationship, with purification percentages estimated at 98.15 ± 0.9% and 97.046 ± 0.47% and removal efficiency of 12.68 ± 0.91 and 15.55 ± 0.72 mg/g for W. anomalus WO2 and R. mucilaginosa RO7, respectively. The investigated metal-tolerant yeast strains proved to be promising candidates for bioremediation processes of heavy metals. This work amends the metal-resistant yeast bank with new strains having interesting abilities to resist to relatively high concentrations of toxic heavy metals and which can be used in the near future as low-cost biosorbents.

从被称为铅和镉的高度污染地区的 Dayet Oum Ghellaz 湖水（阿尔及利亚西北部）中分离和选择了七种金属抗性酵母菌株。根据对七种重金属Hg、Cr、Cd、Pb、Cu、Zn和Fe的抗性筛选酵母菌株，并通过分子和系统发育分析对其进行表征。对 26S rRNA 基因的 D1/D2 结构域的测序揭示了 7 种酵母分离物与粘液红酵母菌、卢西塔氏棒孢菌和异常柳杆菌属的隶属关系。物种。所有酵母菌株都具有耐盐性，因为它们能够在 10-15% NaCl 中生长。酵母分离物对所研究的重金属具有高度抗性，并且根据金属类型表现出不同的耐受性。在R. mucilaginosa RO7 和W. anomalus WO2 菌株中观察到最高的最小抑制浓度 (MIC)，然后选择它们进行铅去除测定。本研究首次调查了W. anomalus对铅的消除作用。铅的吸收受到生物量浓度的显着影响，呈反向关系，W. anomalus WO2 的净化百分比估计为 98.15 ± 0.9% 和 97.046 ± 0.47%，去除效率分别为 12.68 ± 0.91 和 15.55 ± 0.72 mg/g 和R. mucilaginosa RO7，分别。研究的耐金属酵母菌株被证明是重金属生物修复过程的有希望的候选者。这项工作用新菌株修正了金属抗性酵母库，这些菌株具有有趣的抵抗相对高浓度有毒重金属的能力，并且可以在不久的将来用作低成本的生物吸附剂。