Pacific abalone (Haliotis discus hannai) is a commercially important high valued molluscan species. Its wild population has decreased in recent years. It is widely cultured in Korea. Traditional breeding programs have been implemented for hatchery production of abalone seeds. To obtain more genetic information for its molecular breeding program, a high-density linkage map and quantitative trait locus (QTL) for three growth-related traits was constructed for Pacific abalone. F1 cross population with two parents were sampled to construct the linkage map using genotyping by sequencing (GBS). A total of 664,630,534 clean reads and 56,686 SNPs were generated and 3,345 segregating SNPs were used to construct a consensus linkage map. The map spanned 1,747.023 cM with 18 linkage groups and an average interval of 0.55 cM. QTL analysis revealed two significant QTL in LG10 on the consensus linkage map of each growth-related trait. Both QTLs were located in the telomere region of the chromosome. Moreover, four potential candidate genes for growth-related traits were identified in the QTL region. Expression analysis revealed that these identified genes are involved in growth regulation of abalone. The newly constructed genetic linkage map, growth-related QTLs and potential candidate genes identified in the present study can be used as valuable genetic resources for marker-assisted selection (MAS) of Pacific abalone in molecular breeding program.

中文翻译：

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基于SNP标记的遗传连锁图谱构建、QTL图谱构建及太平洋鲍鱼生长相关性状候选基因的基因分型检测

太平洋鲍鱼（Haliotis discus hannai）是一种具有重要商业价值的高价值软体动物物种。近年来，其野生种群数量有所减少。它在韩国被广泛种植。鲍鱼苗孵化场生产采用了传统育种计划。为了为其分子育种计划获得更多遗传信息，构建了太平洋鲍鱼三个生长相关性状的高密度连锁图和数量性状基因座（QTL）。使用基因分型测序 (GBS) 对具有两个亲本的 F1 交叉种群进行采样以构建连锁图。总共生成了 664,630,534 个干净读数和 56,686 个 SNP，并使用 3,345 个分离 SNP 构建了一致连锁图。该图跨越 1,747.023 cM，具有 18 个连锁群，平均间隔为 0.55 cM。QTL 分析显示 LG10 中每个生长相关性状的共有连锁图上有两个显着的 QTL。两个 QTL 都位于染色体的端粒区域。此外，在 QTL 区域鉴定了四个潜在的生长相关性状候选基因。表达分析表明，这些鉴定出的基因参与了鲍鱼的生长调节。本研究新构建的遗传连锁图谱、生长相关QTL和潜在候选基因可作为分子育种计划中太平洋鲍鱼标记辅助选择（MAS）的宝贵遗传资源。在 QTL 区域鉴定了四个潜在的生长相关性状候选基因。表达分析表明，这些鉴定出的基因参与了鲍鱼的生长调节。本研究新构建的遗传连锁图谱、生长相关QTL和潜在候选基因可作为分子育种计划中太平洋鲍鱼标记辅助选择（MAS）的宝贵遗传资源。在 QTL 区域鉴定了四个潜在的生长相关性状候选基因。表达分析表明，这些鉴定出的基因参与了鲍鱼的生长调节。本研究新构建的遗传连锁图谱、生长相关QTL和潜在候选基因可作为分子育种计划中太平洋鲍鱼标记辅助选择（MAS）的宝贵遗传资源。