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Molecular detections of coronavirus: current and emerging methodologies
Expert Review of Anti-infective Therapy ( IF 5.7 ) Pub Date : 2021-07-21 , DOI: 10.1080/14787210.2021.1949986
Mingkun Diao 1 , Lang Lang 1 , Juan Feng 1 , Rongsong Li 1
Affiliation  

ABSTRACT

Introduction

Seven coronavirus species have been identified that can infect humans. While human coronavirus infections had been historically associated with only mild respiratory symptoms similar to the common cold, three coronaviruses identified since 2003, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and SARS-CoV-2, cause life-threatening severe respiratory syndromes. The coronavirus disease 2019 (COVID-19) caused by the highly transmissible SARS-CoV-2 has triggered a worldwide health emergency. Due to the lack of effective drugs and vaccination, rapid and reliable detection is of vital importance to control coronavirus epidemics/pandemics.

Area covered

A literature search was performed in Pubmed covering the detections and diagnostics of SARS, MERS and SARS-CoV-2. This review summarized the current knowledge of established and emerging methods for coronavirus detection. The characteristics of different diagnostic approaches were described, and the strengths and weaknesses of each method were analyzed and compared. In addition, future trends in the field of coronavirus detection were also discussed.

Expert opinion

Nucleic acid–based RT-PCR is the current golden-standard of coronavirus detection, while immunoassays provide history of coronavirus infection besides diagnostic information. Integrated high-throughput system holds the great potential and is the trend of future detection and diagnosis of virus infection.



中文翻译:

冠状病毒的分子检测:当前和新兴的方法

摘要

介绍

已经确定了七种可以感染人类的​​冠状病毒。虽然人类冠状病毒感染在历史上只与类似于普通感冒的轻微呼吸道症状相关,但自 2003 年以来发现了三种冠状病毒,即严重急性呼吸综合征冠状病毒 (SARS-CoV)、中东呼吸综合征冠状病毒 (MERS-CoV) 和 SARS- CoV-2,导致危及生命的严重呼吸系统综合症。由高度传染性的 SARS-CoV-2 引起的 2019 年冠状病毒病 (COVID-19) 引发了全球卫生紧急情况。由于缺乏有效的药物和疫苗接种,快速可靠的检测对于控制冠状病毒流行/大流行至关重要。

覆盖面积

在 Pubmed 中进行了文献检索,涵盖了 SARS、MERS 和 SARS-CoV-2 的检测和诊断。这篇综述总结了当前对已建立和新兴的冠状病毒检测方法的了解。描述了不同诊断方法的特点,并对每种方法的优缺点进行了分析和比较。此外,还讨论了冠状病毒检测领域的未来趋势。

专家意见

基于核酸的 RT-PCR 是当前检测冠状病毒的黄金标准,而免疫分析除了提供诊断信息外,还提供冠状病毒感染的历史。集成的高通量系统具有巨大的潜力,是未来病毒感染检测和诊断的趋势。

更新日期:2021-07-21
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