In this study, the mechanism that vitamin C (VC) regulates the production of reactive oxygen species (ROS) through Wnt10b signaling was investigated in the gill of zebrafish (Danio rerio). The results showed that 0.5 and 1.0 g/kg VC diets induced the gene expression of Wnt10b, β-catenin, SOD, CAT, and GSH-PX in gill. In addition, VC decreased the levels of H₂O₂, O₂^·− and ·OH, whereas the activities of SOD, CAT, and GSH-PX were increased by VC in the gill of zebrafish. To evaluate the role of Wnt10b in regulating oxidative stress, Wnt10b RNA was further interfered and the gene expression and activities of antioxidant enzymes were detected in gill. The result of Wnt10b RNA interference showed that Wnt10b signaling played a key role in regulating the gene expression of SOD, CAT, and GSH-PX. In all, VC may regulate the production of ROS through Wnt10b signaling in the gill of zebrafish (Danio rerio).

在这项研究中，在斑马鱼 ( Danio rerio )的鳃中研究了维生素 C (VC) 通过 Wnt10b 信号传导调节活性氧 (ROS) 产生的机制。结果表明，0.5和1.0 g/kg VC日粮诱导了鳃中Wnt10b、β-catenin、SOD、CAT和GSH-PX的基因表达。此外，VC降低了H ₂ O ₂、O ₂ ^·-和·OH，而VC增加了斑马鱼鳃中SOD、CAT和GSH-PX的活性。为了评估Wnt10b在调节氧化应激中的作用，进一步干扰Wnt10b RNA并检测鳃中抗氧化酶的基因表达和活性。Wnt10b RNA干扰结果表明Wnt10b信号在调控SOD、CAT和GSH-PX基因表达中起关键作用。总之，VC 可能通过 Wnt10b 信号在斑马鱼 ( Danio rerio )的鳃中调节 ROS 的产生。