Calcium phosphate (CaP) nanomaterials are considered as potential biomaterial for drug delivery systems because of their excellent biocompatible features. In the present study, amino glycoside antibiotics, such as kanamycin and gentamycin-loaded CaP nanoparticles, were successfully synthesized by the precipitation method. Physicochemical properties were analyzed by XRD, FTIR, FE-SEM, EDX, and light-scattering (DLS) measurements. The average size of CaP nanoparticles ranges from 20 to 100 nm, as measured by DLS analysis. The crystallinity of the prepared nanoparticles was confirmed by XRD analysis, which revealed that the CaP nanoparticles were in crystalline and hydroxyapatite (HA) phase with tricalcium phosphate (TCP). The FTIR spectrum confirmed the presence of phosphate and surface hydroxyl groups. In vitro release study of drug-loaded CaP (D-CaP) nanoparticles was performed, and the drug release from nanoparticles was sustained over 5 days, with an entrapment efficiency of 52.05 ± 3% and 65.3 ± 4%, respectively. The antibacterial activity of D-CaP nanoparticles was evaluated against Bacillus cereus (KACC 14394), Staphylococcus aureus (KCTC 1916) Escherichia coli (KACC 10005), and Salmonella typhi (KCCM 40253) by the well diffusion and minimum inhibitory concentration (MIC) methods. We found the bactericidal effect of D-CaP nanoparticles in a dose-dependent manner by MTT assay. In addition, the interaction of D-CaP nanoparticles against bacterial pathogens was demonstrated by Bio-TEM analysis, providing clear evidence of the disruption of bacterial cell membranes. The results of the present study suggest that D-CaP nanoparticles can be useful for the treatment of bacterial infection in the bone as well as in the biomedical field.

磷酸钙（CaP）纳米材料由于其优异的生物相容性特性而被认为是用于药物递送系统的潜在生物材料。在本研究中，通过沉淀法成功合成了氨基糖苷类抗生素，如卡那霉素和庆大霉素负载的 CaP 纳米颗粒。通过 XRD、FTIR、FE-SEM、EDX 和光散射 (DLS) 测量分析物理化学性质。根据 DLS 分析测量，CaP 纳米颗粒的平均尺寸范围为 20 到 100 nm。XRD 分析证实了制备的纳米粒子的结晶度，这表明 CaP 纳米粒子处于结晶和羟基磷灰石 (HA) 相与磷酸三钙 (TCP) 相。FTIR 光谱证实了磷酸盐和表面羟基的存在。进行了载药CaP（D-CaP）纳米颗粒的体外释放研究，纳米颗粒的药物释放持续5天以上，包封率分别为52.05±3%和65.3±4%。D-CaP纳米颗粒的抗菌活性评估蜡状芽孢杆菌(KACC 14394)、金黄色葡萄球菌(KCTC 1916)大肠杆菌(KACC 10005) 和伤寒沙门氏菌(KCCM 40253) 通过井扩散和最小抑制浓度 (MIC) 方法。我们通过 MTT 测定发现 D-CaP 纳米颗粒的杀菌作用呈剂量依赖性。此外，生物 TEM 分析证明了 D-CaP 纳米颗粒与细菌病原体的相互作用，为细菌细胞膜的破坏提供了明确的证据。本研究的结果表明，D-CaP 纳米颗粒可用于治疗骨骼和生物医学领域的细菌感染。