Objective

To determine the relationship between arterial blood colour [as defined by the International Commission on Illumination (CIE) L∗a∗b∗ colour space] and haemoglobin oxygen saturation [functional saturation (SaO₂) and fractional saturation (FO₂Hb)], and if arterial blood colour can be used to predict arterial haemoglobin oxygen saturation.

Study design

Descriptive study as an adjunct to two prospective randomized crossover studies.

Animals

A group of 10 wild caught adult female impala (Aepyceros melampus) weighing 34.1 ± 5.2 kg (mean ± standard deviation).

Methods

Impala were immobilized with potent opioids (0.09 mg kg^–1 of etorphine or thiafentanil). A total of 163 arterial blood samples were collected anaerobically into heparinized syringes from arterial cannulae and analysed immediately using spectrocolourimetry and co-oximetry. Data were analysed by modelling the relationship between predicted arterial blood colour CIE L∗a∗b∗ components and SaO₂ and FO₂Hb. The models were then used to predict values for L∗, a∗ and b∗ to produce a colour palette for the range of SaO₂ and FO₂Hb used. The modified version of the Farnsworth-Munsell hue test was used to assess the subjective ordering of the resulting colour palette by 20 observers.

Results

The second-order polynomial (quadratic) model produced the best fit for all three arterial blood colour CIE L∗a∗b∗ components for both SaO₂ and FO₂Hb. The regression models were used to generate predicted arterial blood colour CIE L∗a∗b∗ components for the midpoint of each decile over a range of SaO₂ and FO₂Hb percentages (15% to 95%). The resulting colour palettes were correctly ordered by all observers in the SaO₂ range of 45–95% saturation.

Conclusions and clinical relevance

An association between arterial blood colour (as defined by CIE L∗a∗b∗ components) and SaO₂ and FO₂Hb exists, and arterial blood colour can be used to give a clinically useful estimate of arterial haemoglobin oxygen saturation in impala.

中文翻译：

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使用血色评估固定黑斑羚 (Aepyceros melampus) 的动脉血氧饱和度

客观的

为了确定动脉血色 [由国际照明委员会 (CIE) L∗a∗b∗ 颜色空间定义] 和血红蛋白氧饱和度 [功能饱和度 (SaO ₂ ) 和分数饱和度 (FO ₂ Hb)] 之间的关系，以及是否可以使用动脉血色来预测动脉血红蛋白氧饱和度。

学习规划

描述性研究作为两项前瞻性随机交叉研究的补充。

动物

一组 10 只野生捕获的成年雌性黑斑羚 ( Aepyceros melampus )，体重 34.1 ± 5.2 公斤（平均值 ± 标准差）。

方法

Impala 用强效阿片类药物（0.09 mg kg ^–1的埃托啡或噻芬太尼）进行固定。总共 163 份动脉血样本从动脉插管厌氧收集到肝素化注射器中，并立即使用分光比色法和共氧测定法进行分析。通过模拟预测的动脉血色 CIE L*a*b* 成分与 SaO ₂和 FO ₂ Hb 之间的关系来分析数据。_{然后使用这些模型来预测 L*、a* 和 b* 的值，以生成适用于所用 SaO 2}和 FO ₂ Hb范围的调色板。Farnsworth-Munsell 色调测试的修改版本用于评估 20 名观察者对所得调色板的主观排序。

结果

二阶多项式（二次）模型对 SaO ₂和 FO ₂ Hb 的所有三种动脉血色 CIE L∗a∗b∗ 分量产生了最佳拟合。_{回归模型用于在 SaO 2}和 FO ₂ Hb 百分比（15% 至 95%）范围内为每个十分位数的中点生成预测的动脉血颜色 CIE L*a*b* 分量。_{在 SaO 2}饱和度为 45-95%的范围内，所有观察者都正确排序了生成的调色板。

结论和临床相关性

动脉血色（由 CIE L*a*b* 成分定义）与 SaO ₂和 FO ₂ Hb 之间存在关联，并且动脉血色可用于对黑斑羚的动脉血红蛋白氧饱和度进行临床有用的估计。