Abstract

Our study aimed to evaluate the effects of Gal-1 in dose depending manner on maturation and immunomodulatory properties of monocyte-derived (Mo) DCs in-vitro. The effects were analyzed by monitoring their phenotypic characteristics, cytokine profile, and the ability to direct the immune response in the co-culture with allogeneic CD4⁺T cells. Gal-1 reduced the expression of CD80 and CD86 molecules on MoDCs compared to untreated MoDCs. Gal-1 at concentrations of 1 and 6 μg/mL significantly reduced IL-12 production, while the concentration of 3 μg/mL led to its significant increase. Gal-1 in all concentrations induced a significant increase in the production of IL-10. Treatment of MoDCs with 3 and 6 μg/mL of Gal-1 stimulated the production of IL-2 and IFN-γ in the co-culture with CD4⁺T lymphocytes. This study demonstrated a dual immunomodulatory effect of Gal-1 on MoDCs in terms of immune stimulation and immune suppression, depending on the applied concentration.

摘要

我们的研究旨在评估 Gal-1 对体外单核细胞衍生 (Mo) DCs 成熟度和免疫调节特性的影响。通过监测它们的表型特征、细胞因子谱以及在与同种异体 CD4 ⁺ T 细胞共培养中指导免疫反应的能力来分析这些影响。与未经处理的 MoDC 相比，Gal-1 降低了 MoDC 上 CD80 和 CD86 分子的表达。浓度为 1 和 6 μg/mL 的 Gal-1 显着降低了 IL-12 的产生，而 3 μg/mL 的浓度导致其显着增加。所有浓度的 Gal-1 均诱导 IL-10 的产生显着增加。用 3 和 6 μg/mL 的 Gal-1 处理 MoDC 刺激了与 CD4 共培养的 IL-2 和 IFN-γ 的产生⁺ T 淋巴细胞。这项研究证明了 Gal-1 在免疫刺激和免疫抑制方面对 MoDC 的双重免疫调节作用，具体取决于应用的浓度。