Microbial bioremediation of oil-contaminated sites is still a challenge due to the slower rate and susceptibility of microbes to a higher concentration of oil. The poor bioavailability, hydrophobicity, and non-polar nature of oil slow down microbial biodegradation. In this study, biodegradation of crude oil is performed in fed-batch mode using an oil-degrader Pseudomonas aeruginosa to address the issue of substrate toxicity. The slower biodegradation was integrated with faster biosorption for effective oil remediation. Highly fibrous and porous sugarcane bagasse was surface modified with hydrophobic octyl groups to improve the surface-oil interactions. The microbe showed 2 folds enhanced oil degradation in the fed-batch study, which was further increased by 1·5 folds in the integrated biosorption coupled biodegradation approach. The biosorption-assisted biodegradation approach supported the microbial growth to 2 folds higher than the fed-batch study without biosorbent. The analysis of biosurfactant production indicated the 3 folds higher concentration in fed-batch modes as compared to batch study. In the integrated strategy, the concentration of contaminant (oil) reduces to quite a tolerable level to microbes, which improved effective metabolism and thus overall biodegradation. This study puts forward a promising strategy for improved degradation of hazardous hydrophobic contaminants in a sustainable, economic and eco-friendly manner.

中文翻译：

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以补料分批模式整合生物吸附和生物降解以加强原油修复

由于微生物对较高浓度油的较慢速度和易感性，油污染场地的微生物生物修复仍然是一个挑战。油的生物利用度差、疏水性和非极性特性会减缓微生物的生物降解。在这项研究中，原油的生物降解是使用油降解剂铜绿假单胞菌以分批补料模式进行的解决底物毒性问题。较慢的生物降解与较快的生物吸附相结合，以实现有效的石油修复。高度纤维化和多孔的甘蔗渣用疏水性辛基进行表面改性，以改善表面-油的相互作用。微生物在补料分批研究中显示出 2 倍的油降解增强，在集成的生物吸附耦合生物降解方法中进一步增加了 1·5 倍。生物吸附辅助的生物降解方法支持的微生物生长比没有生物吸附剂的补料分批研究高 2 倍。生物表面活性剂生产的分析表明，与分批研究相比，补料分批模式的浓度高 3 倍。在综合策略中，污染物（油）的浓度降低到微生物可以容忍的水平，这改善了有效代谢，从而改善了整体生物降解。这项研究提出了一种有前景的策略，以可持续、经济和生态友好的方式改善有害疏水污染物的降解。