The environmental oxygen concentration is a crucial factor affecting cell proliferation. Owing to the reversible binding property of hemoglobin to oxygen, it can be utilized to regulate the oxygen concentration in vitro and its ability to reduce apoptosis can be evaluated. In this study, a process comprising isolation, purification, and extraction was used to obtain hemoglobin from Perinereis aibuhitensis, a polychaete invertebrate. Extracts were separated and characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Extract component identity was confirmed by matrix-assisted laser desorption time-of-flight mass spectrometry analysis, with the molecular weight determined as 412216.6875 Da. The oxygen carrying capacity of P. aibuhitensis hemoglobin was approximately comparable to that of human hemoglobin. P. aibuhitensis hemoglobin remarkably downregulated the apoptosis rate. Reactive oxygen species (ROS) assays confirmed the reduction in ROS production, enabling a better elucidation of the mechanism underlying the decrease in apoptosis. These results suggested that P. aibuhitensis hemoglobin is a natural oxygen carrier, that, owing to its low-cost and accessibility, can be considered a candidate culture medium supplement to reduce the apoptosis rate.

中文翻译：

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从 Perinereis aibuhitensis 中提取的血红蛋白作为细胞培养基补充剂以减少细胞凋亡

环境氧浓度是影响细胞增殖的关键因素。由于血红蛋白与氧的可逆结合特性，它可用于调节体外氧浓度并评估其减少细胞凋亡的能力。在这项研究中，使用包括分离、纯化和提取的过程从多毛类无脊椎动物 Perinereis aibuhitensis 中获取血红蛋白。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离和表征提取物。通过基质辅助激光解吸飞行时间质谱分析确认提取物组分身份，分子量确定为 412216.6875 Da。P. aibuhitensis 血红蛋白的携氧能力与人类血红蛋白的携氧能力大致相当。P。aibuhitensis 血红蛋白显着下调细胞凋亡率。活性氧 (ROS) 测定证实了 ROS 产生的减少，从而能够更好地阐明细胞凋亡减少的潜在机制。这些结果表明 P. aibuhitensis 血红蛋白是一种天然氧载体，由于其低成本和可及性，可以被认为是降低细胞凋亡率的候选培养基补充剂。