Quorum sensing (QS) offers cell density dependent dynamic regulations in cell culture through devices such as synchronized lysis circuit (SLC) and metabolic toggle switch (MTS). However, there is still a lack of studies on cocultivation with a combination of different QS-based devices. Taking the production of isopropanol and salidroside as case studies, we have mathematically modeled a comprehensive set of QS-regulated cocultivation schemes and constructed experimental combinations of QS devices, respectively, to evaluate their feasibility and optimality for regulating growth competition and corporative production. Glucose split ratio is proposed for the analysis of competition between cell growth and targeted production. Results show that the combination of different QS devices across multiple members offers a new tool with the potential to effectively coordinate synthetic microbial consortia for achieving high product titer in cross-feeding cocultivation. It is also evident that the performance of such systems is significantly affected by dynamic characteristics of chosen QS devices, carbon source control and the operational settings. This study offers insights for future applications of combinational QS devices in synthetic microbial consortia.

群体感应 (QS) 通过同步裂解电路 (SLC) 和代谢切换开关 (MTS) 等设备在细胞培养中提供依赖于细胞密度的动态调节。然而，仍然缺乏对不同基于 QS 的设备组合进行共培养的研究。以异丙醇和红景天苷的生产为例，我们对一套综合的 QS 调节共培养方案进行了数学建模，并分别构建了 QS 装置的实验组合，以评估其调节生长竞争和企业生产的可行性和最优性。建议葡萄糖分流比用于分析细胞生长和靶向生产之间的竞争。结果表明，跨多个成员的不同 QS 设备的组合提供了一种新工具，具有有效协调合成微生物联盟的潜力，以在交叉饲养共培养中实现高产品滴度。同样明显的是，此类系统的性能受所选 QS 设备的动态特性、碳源控制和操作设置的显着影响。这项研究为组合 QS 设备在合成微生物聚生体中的未来应用提供了见解。