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Growth inhibition of phytopathogenic spiroplasmas by membrane-interactive antimicrobial peptides Novispirin T7 and Caerin 1.1
Annals of Applied Biology ( IF 2.6 ) Pub Date : 2021-07-02 , DOI: 10.1111/aab.12715
Wei Wei 1 , Robert E. Davis 1 , Joseph D. Mowery 2 , Yan Zhao 1
Affiliation  

Spiroplasma kunkelii and Spiroplasma citri, both helical-shaped cell wall-less bacteria, are the causative agents of corn stunt disease and citrus stubborn disease, respectively. Plants exhibiting natural resistance to these phytopathogenic spiroplasmas are currently lacking. Engineering artificial plant resistance using antimicrobial peptides (AMPs) has been conceived as a new approach to control the agronomically important spiroplasmal diseases. In preparation for such task, the present study focused on screening of AMPs that have potentials to curb the growth of S. kunkelii and S. citri. Four AMPs, including Novispirin T7, Caerin 1.1, Tricholongin and Dhvar4, were selected for in vitro growth inhibition test. A liquid assay method was developed for quick qualitative and quantitative evaluations of the AMPs. Our results demonstrated that Novispirin T7 and Caerin 1.1 were able to inhibit the growth of both phytopathogenic spiroplasmas with the efficacy comparable to that of tetracycline. Cell deformations were observed in spiroplasma cultures treated with these two peptides, indicating interactions of the AMPs with the spiroplasma cell membranes. The minimum inhibitory concentrations (MICs) of the AMPs against S. kunkelii and S. citri were determined.

中文翻译:

膜相互作用抗菌肽 Novispirin T7 和 Caerin 1.1 抑制植物病原性螺旋体的生长

Spiroplasma kunkeliiSpiroplasma citri均为螺旋形细胞壁无壁细菌,分别是玉米矮化病和柑桔顽固病的病原体。目前缺乏对这些植物病原螺旋体表现出天然抗性的植物。使用抗微生物肽 (AMP) 设计人工植物抗性已被认为是控制农艺学上重要的螺旋体病害的新方法。为准备这项任务,本研究的重点是筛选具有抑制S. kunkeliiS. citri生长潜力的 AMPs. 选择四种AMPs,包括Novispirin T7、Caerin 1.1、Tricholongin和Dhvar4,用于体外生长抑制试验。开发了一种液体测定方法,用于对 AMP 进行快速定性和定量评估。我们的结果表明,Novispirin T7 和 Caerin 1.1 能够抑制两种植物致病性螺旋体的生长,其功效与四环素相当。在用这两种肽处理的螺旋体培养物中观察到细胞变形,表明 AMP 与螺旋体细胞膜的相互作用。确定了 AMP 对S. kunkeliiS. citri的最小抑制浓度 (MIC) 。
更新日期:2021-07-02
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