Adropin (ADR) plays a role in metabolism regulation and its alterations in obesity and diabetes have been found. Treatment with ADR was beneficial in metabolic diseases, and physical exercise increased ADR concentrations in obese patients. However, data on the distribution of ADR in the brain are sparse. The role of metabolic status and physical exercise on its expression in the brain is undiscovered.

We hypothesized that diabetes type 2 (DM2) and/or exercise will alter number of ADR-immunoractive (-ir) cells in the rat brain.

Animals were divided into groups: diabetes type 2 (receiving high-fat diet and injections of streptozotocin) and control (fed laboratory chow diet; C). Rats were further divided into: running group (2 weeks of forced exercise on a treadmill) and non-running group. Body mass, metabolic and hormonal profiles were assessed. Immunohistochemistry was run to study ADR-ir cells in the brain.

We found that: 1) in DM2 animals, running decreased insulin and increased glucose concentrations; 2) in C rats, running decreased insulin concentrations and had no effect on glucose concentration in blood; 3) running increased corticosterone (CORT) concentrations in DM2 and C rats; 4) ADR-ir cells were detected in the hippocampus and ADR-ir fibers in the arcuate nucleus of the hypothalamus, which is a novel location; 5) metabolic status and running, however, did not change number of these cells.

We concluded that 2 weeks of forced moderate intensity locomotor training induced stress response present as increased concentration of CORT and did not influence number of ADR-ir cells in the brain.

Adropin (ADR) 在代谢调节中发挥作用，并且已发现其对肥胖和糖尿病的改变。ADR 治疗对代谢性疾病有益，体育锻炼可增加肥胖患者的 ADR 浓度。然而，关于 ADR 在大脑中分布的数据很少。代谢状态和体育锻炼对其在大脑中表达的作用尚未被发现。

我们假设 2 型糖尿病 (DM2) 和/或运动会改变大鼠脑中 ADR 免疫活性 (-ir) 细胞的数量。

动物被分成两组：2型糖尿病（接受高脂肪饮食和注射链脲佐菌素）和对照组（喂食实验室食物；C）。大鼠进一步分为：跑步组（在跑步机上强制锻炼2周）和非跑步组。评估了体重、代谢和激素谱。运行免疫组织化学来研究大脑中的 ADR-ir 细胞。

我们发现： 1) 在 DM2 动物中，跑步降低了胰岛素和增加了葡萄糖浓度；2）在C大鼠中，跑步降低了胰岛素浓度，对血液中的葡萄糖浓度没有影响；3) 在 DM2 和 C 大鼠中增加皮质酮 (CORT) 浓度；4）在海马和下丘脑弓状核的ADR-ir纤维中检测到ADR-ir细胞，这是一个新的位置；5）代谢状态和跑步，然而，并没有改变这些细胞的数量。

我们得出结论，2 周的强迫中等强度运动训练诱导的应激反应表现为 CORT 浓度增加，并且不影响大脑中 ADR-ir 细胞的数量。