The goal of this investigation was to establish a reliable technique for the quantitation of ponazuril in limited sample volumes. Samples were extracted with chloroform and separation was achieved with a Symmetry RP₁₈ column. Ultraviolet absorption was measured at 254 nm. A combination of 0.1% formic acid and acetonitrile (50:50) was used as the mobile phase. The calibration curve was linear from 0.1–25 μg/mL, with a lower limit of quantification of 0.1 μg/mL with a 100 μL sample. The precision and accuracy were well within the range set by the Food and Drug Administration and the recovery was over 95%. This technique was used to analyze ponazuril samples and found to be appropriate for pharmacokinetic studies.

中文翻译：

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测定血浆中泊那珠利的生物分析反相高效液相色谱法

这项研究的目的是建立一种可靠的技术，用于在有限的样品体积中定量泊那珠利。样品用氯仿萃取，并用 Symmetry RP ₁₈色谱柱实现分离。在 254 nm 处测量紫外吸收。使用 0.1% 甲酸和乙腈 (50:50) 的组合作为流动相。校准曲线在 0.1–25 μg/mL 范围内呈线性，100 μL 样品的定量下限为 0.1 μg/mL。精密度和准确度均在食品药品监督管理局规定的范围内，回收率达95%以上。该技术用于分析泊那珠利样品，发现适用于药代动力学研究。