当前位置: X-MOL 学术Euphytica › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genome-wide association studies for sulfur-containing amino acids in soybean seeds
Euphytica ( IF 1.9 ) Pub Date : 2021-07-03 , DOI: 10.1007/s10681-021-02888-8
Wenjie Yuan 1 , Zhiyi Wu 1 , Yu’e Zhang 1 , Rufei Yang 1 , Hui Wang 1 , Guizhen Kan 1 , Deyue Yu 1, 2
Affiliation  

Soybean provides abundant dietary proteins for humans and livestock. However, the low content of sulfur-containing amino acids [SAA, including methionine (Met) and cysteine (Cys)] in soybean protein limits the quality and prevents it from becoming an ideal food and feed ingredient. Thus, the exploration of novel high-SAA soybean germplasm and identification of related genes is necessary. In this study, a genome-wide association study (GWAS) was conducted for Cys, Met and SAA in 165 soybean materials genotyped with a high-density SNP array. A total of 138 significant SNPs associated with three traits were identified. Moreover, one SNP on chromosome 7 was identified in three environments. Glyma.07g175700 and Glyma.07g176000 at the LD of AX-94036794 were considered candidate genes. Quantitative real-time PCR showed that different expression levels of these genes were observed in high-SAA and low-SAA material, which suggested that these two genes may be involved in SAA synthesis. These results will help to increase our understanding of the genetic mechanisms of SAA and improve nutritional quality through molecular marker-assisted selection breeding in soybeans.



中文翻译:

大豆种子中含硫氨基酸的全基因组关联研究

大豆为人类和牲畜提供丰富的膳食蛋白质。然而,大豆蛋白中含硫氨基酸[SAA,包括蛋氨酸(Met)和半胱氨酸(Cys)]含量低,限制了其质量,使其无法成为理想的食品和饲料成分。因此,探索新的高SAA大豆种质和相关基因的鉴定是必要的。在这项研究中,对使用高密度 SNP 阵列进行基因分型的 165 种大豆材料中的 Cys、Met 和 SAA 进行了全基因组关联研究 (GWAS)。共鉴定了 138 个与三个性状相关的重要 SNP。此外,在三种环境中鉴定了 7 号染色体上的一个 SNP。Glyma.07g175700Glyma.07g176000在 AX-94036794 的 LD 处被认为是候选基因。定量实时PCR显示,在高SAA和低SAA材料中观察到这些基因的不同表达水平,这表明这两个基因可能参与了SAA合成。这些结果将有助于增加我们对 SAA 遗传机制的理解,并通过分子标记辅助选择育种改善大豆的营养质量。

更新日期:2021-07-04
down
wechat
bug