Significance: Most cases of high-grade serous ovarian carcinoma originate as serous tubal intraepithelial carcinoma (STIC) lesions in the fallopian tube epithelium (FTE), enabling early endoscopic detection. Aim: The cell-acquiring fallopian endoscope (CAFE) was built to meet requirements for locating potentially pathological tissue indicated by an alteration in autofluorescence or presence of a targeted fluorophore. A channel was included for directed scrape biopsy of cells from regions of interest. Approach: Imaging resolution and fluorescence sensitivity were measured using a standard resolution target and fluorescence standards, respectively. A prototype was tested in ex vivo tissue, and collected cells were counted and processed. Results: Measured imaging resolution was 88 μm at a 5-mm distance, and full field of view was ∼45 deg in air. Reflectance and fluorescence images in ex vivo porcine reproductive tracts were captured, and fit through human tracts was verified. Hemocytometry counts showed that on the order of 105 cells per scrape biopsy could be collected from ex vivo porcine tissue. Conclusions: All requirements for viewing STIC in the FTE were met, and collected cell counts exceeded input requirements for relevant analyses. Our benchtop findings suggest the potential utility of the CAFE device for in vivo imaging and cell collection in future clinical trials.

中文翻译：

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亚毫米内窥镜证明了输卵管中体内反射成像、荧光成像和细胞收集的可行性

意义：大多数高级别浆液性卵巢癌病例起源于输卵管上皮 (FTE) 中的浆液性输卵管上皮内癌 (STIC) 病变，可以进行早期内镜检测。目的：细胞获得性输卵管内窥镜 (CAFE) 旨在满足定位潜在病理组织的要求，这些组织由自发荧光的改变或目标荧光团的存在指示。包括一个通道，用于对感兴趣区域的细胞进行定向刮擦活检。方法：分别使用标准分辨率目标和荧光标准来测量成像分辨率和荧光灵敏度。在离体组织中测试原型，并对收集的细胞进行计数和处理。结果：在 5 毫米距离处测得的成像分辨率为 88 微米，全视场在空气中约为 45 度。捕获了离体猪生殖道中的反射和荧光图像，并验证了其是否适合人体生殖道。血细胞计数显示，每次刮取活检可以从离体猪组织中收集大约 105 个细胞。结论：满足在 FTE 中查看 STIC 的所有要求，并且收集的细胞计数超过了相关分析的输入要求。我们的台式研究结果表明 CAFE 设备在未来临床试验中用于体内成像和细胞收集的潜在效用。满足在 FTE 中查看 STIC 的所有要求，并且收集的细胞计数超过了相关分析的输入要求。我们的台式研究结果表明 CAFE 设备在未来临床试验中用于体内成像和细胞收集的潜在效用。满足在 FTE 中查看 STIC 的所有要求，并且收集的细胞计数超过了相关分析的输入要求。我们的台式研究结果表明 CAFE 设备在未来临床试验中用于体内成像和细胞收集的潜在效用。