The determination and separation of enantiomers is an interesting and important topic of research in various fields, e.g., biochemistry, food science, pharmaceutical industry, environment, etc. Although these compounds possess identical physicochemical properties, a pair of enantiomers often has different pharmacological, toxicological, and metabolic activities. For this reason, chiral discrimination by using chromatographic and electromigration techniques has become an urgent need in the pharmaceutical field. This review intends to offer the “state of the art” about the separation of chiral antifungal drugs and several related precursors by both liquid and gas chromatography, as well as electromigration methods. This overview is organized into two sections. The first one describes general considerations on chiral antifungal drugs. The second part deals with the main analytical methods for the enantiomeric discrimination of these drugs, including a brief description of chiral selectors and stationary phases. Moreover, many recent applications attesting the great interest of analytical chemists in the field of enantiomeric separation are presented.

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通过色谱和电迁移技术手性分离和分析抗真菌药物：2010-2020 年取得的结果

对映体的测定和分离是生物化学、食品科学、制药工业、环境等各个领域的一个有趣且重要的研究课题。虽然这些化合物具有相同的理化性质，但一对对映体往往具有不同的药理、毒理学, 和代谢活动。为此，利用色谱和电迁移技术进行手性鉴别已成为制药领域的迫切需求。本综述旨在提供有关通过液相色谱和气相色谱以及电迁移方法分离手性抗真菌药物和几种相关前体的“最新技术”。本概述分为两个部分。第一个描述了手性抗真菌药物的一般注意事项。第二部分介绍了这些药物对映体鉴别的主要分析方法，包括手性选择剂和固定相的简要说明。此外，许多最近的应用证明了分析化学家在对映体分离领域的极大兴趣。