Abstract

A simple and an available method is proposed for the determination of quinolone antibiotics using solid-phase fluorescence digital colorimetry. The intrinsic fluorescence of 17 quinolones and the quinolone-sensitizedfluorescence of europium on cellulose paper (CP) and a thin silica layer are studied. Upon the irradiation of quinolone solutions applied to a matrix with ultraviolet light (365 nm), blue (CP, high-performance thin-layer chromatography (HPTLC)) and pink (CP–Eu, HPTLC–Eu) fluorescence is observed. Measurement of the fluorescence intensity on the surface of matrices is carried out using a smartphone. The values of colorimetric parameters in the RGB system are used as the analytical signal (A_r): A_r = \(\sqrt {{{{({{{\text{R}}}_{0}} - {{{\text{R}}}_{{\text{x}}}})}}^{2}} + {{{({{{\text{G}}}_{0}} - {{{\text{G}}}_{{\text{x}}}})}}^{2}} + {{{({{{\text{B}}}_{0}} - {{{\text{B}}}_{{\text{x}}}})}}^{2}}} .\) The limits of detection and determination are 0.2–4 and 0.6–12 µg/mL, respectively, for all analyzed analytes. The analytical ranges are 0.6–500 µg/mL. A procedure for the determination of fluoroquinolones in medicinal preparations is proposed. The relative standard deviation of the results of analysis does not exceed 5%.

中文翻译：

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使用智能手机固相荧光法测定纤维素纸和二氧化硅薄层中药物制剂中的喹诺酮类

摘要

提出了一种使用固相荧光数字比色法测定喹诺酮类抗生素的简单可行的方法。研究了纤维素纸 ( CP ) 和二氧化硅薄层上 17 种喹诺酮类药物的固有荧光和铕的喹诺酮类增感荧光。在用紫外光（365 nm）照射应用于基质的喹诺酮溶液后，观察到蓝色（CP，高效薄层色谱（HPTLC））和粉红色（CP-Eu，HPTLC-Eu）荧光。使用智能手机测量基质表面的荧光强度。RGB 系统中的色度参数值用作分析信号 ( A _r )：A _r =  \(\sqrt {{{{({{{\text{R}}}}_{0}} - {{{\text{R}}}}_{{\text{x}}}})}} ^{2}} + {{{({{{\text{G}}}_{0}} - {{{\text{G}}}}_{{\text{x}}}})}} ^{2}} + {{{({{{\text{B}}}_{0}} - {{{\text{B}}}}_{{\text{x}}}})}} ^{2}}} .\)所有分析物的检测限和测定限分别为 0.2–4 和 0.6–12 µg/mL。分析范围为 0.6–500 µg/mL。提出了一种测定药物制剂中氟喹诺酮类药物的方法。分析结果的相对标准偏差不超过5%。