Notably, a growing number of long noncoding RNAs (lncRNAs) have been recognized to play critical roles in hepatocellular carcinoma (HCC) progression. In this study, we identified a new lncRNA, Linc1749808 (ID: XR_001749808.1) based on microarray data from HCC tissues. Linc1749808 levels in 72 HCC tissues and paracancerous samples were detected by qRT-PCR. The interaction between Linc1749808 and microRNA-206 (miR-206) was assessed by bioinformatic analysis and luciferase assays. Linc1749808 depletion assays, Transwell assays, and miR-206-inhibitor rescue experiments were performed to examine the role of the Linc1749808/miR-206 axis in HCC cells. Our results showed that Linc1749808 was highly expressed in both HCC tissues and cell lines. Linc1749808 expression was significantly correlated with microvascular invasion, metastasis, and prognosis. After the knockdown of Linc1749808, the metastatic potential of 97H and HepG2 cells was attenuated in vitro and in vivo, but the proliferative capacity did not significantly change. Furthermore, Linc1749808 was found to act as a sponge of miR-206. Inhibition of miR-206 counteracted the effect of Linc1749808 knockdown in 97H cells by regulating YAP1 and epithelial-mesenchymal transition (EMT). In summary, these findings show that Linc1749808 can exacerbate the metastasis of HCC by sponging miR-206.

中文翻译：

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新型长链非编码 RNA Linc1749808 通过负调控 miR-206 促进肝细胞癌转移。

值得注意的是，越来越多的长链非编码 RNA (lncRNA) 已被认为在肝细胞癌 (HCC) 进展中发挥关键作用。在本研究中，我们基于来自 HCC 组织的微阵列数据鉴定了一种新的 lncRNA，Linc1749808（ID：XR_001749808.1）。通过 qRT-PCR 检测 72 个 HCC 组织和癌旁样本中的 Linc1749808 水平。通过生物信息学分析和荧光素酶测定评估了 Linc1749808 和 microRNA-206 (miR-206) 之间的相互作用。进行了 Linc1749808 消耗测定、Transwell 测定和 miR-206 抑制剂拯救实验，以检查 Linc1749808/miR-206 轴在 HCC 细胞中的作用。我们的结果表明，Linc1749808 在 HCC 组织和细胞系中均高表达。Linc1749808 表达与微血管侵袭、转移和预后显着相关。敲低Linc1749808后，97H和HepG2细胞的体外和体内转移潜能均减弱，但增殖能力没有明显变化。此外，发现 Linc1749808 充当 miR-206 的海绵。抑制 miR-206 通过调节 YAP1 和上皮间质转化 (EMT) 抵消了 Linc1749808 敲低在 97H 细胞中的作用。总之，这些发现表明，Linc1749808 可通过海绵化 miR-206 加剧 HCC 的转移。抑制 miR-206 通过调节 YAP1 和上皮间质转化 (EMT) 抵消了 Linc1749808 敲低在 97H 细胞中的作用。总之，这些发现表明，Linc1749808 可通过海绵化 miR-206 加剧 HCC 的转移。抑制 miR-206 通过调节 YAP1 和上皮间质转化 (EMT) 抵消了 Linc1749808 敲低在 97H 细胞中的作用。总之，这些发现表明，Linc1749808 可通过海绵化 miR-206 加剧 HCC 的转移。